Background: Acute graft versus host disease (aGVHD) is a common complication following allogeneic hematopoietic stem cell transplantation (AHSCT) caused by cellular andinflammatory factors, including those arising from monocytes and dendritic cells as integral parts of theimmunesystem. Long non-coding RNAs (LNCRNA) have recently emerged as potential regulators of theimmune responses and it is supported that their dysregulation can develop various immune disorders. As an intergenic LNCRNA, the LNC-DC was shown to regulate the human monocytes differentiation and antigen presenting cells (APCs) activation during immune responses. It is also shown that LNC-DC knockdown reduces T-cell activation and cytokine release. Objectives: The aim of this study was to assess whether the LNC-DC plays a role in patients with aGVHD by measuring its expression levels compared to non-aGVHD patients on specific time intervals following transplantation. Methods: Participants included 38 patients who underwent primary allogeneic bone marrow transplantation. Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Hypaque gradient from the blood samples collected at days 0, 7, 14, 28, and final day of transplantation. The qRT-PCR was used to quantify the LNC-DC levels. Results: Findings revealed a significant increase in the LNC-DC levels on day 28 and the final day after transplantation in patients with aGVHD compared to non-GVHD patients (CI = 95%, P < 0. 03 on day 28 and P < 0. 01 on the final day). Furthermore, the receiver operating characteristic (ROC) curve analysis showed an acceptable total area under the curve for the LNC-DC gene expression data, suggesting a fair diagnostic value for LNC-DC. Conclusions: Taken together, data of the present study supported a strong correlation between LNCRNA-DC expression and aGVHD occurrence. As a result, LNC-DC may be considered as a new molecular marker for the aGVHD prognosis.