Horseradish peroxidase was encapsulated in calcium alginate for the purpose of phenol removal. Considering enzyme encapsulation efficiency, retention activity and enzyme leakage of the capsules, the best gelation condition was found to be 1 % w/v of sodium alginate solution and 5.5 % w/v of calcium chloride hexahydrate. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Besides, for each phenol concentration there would be an enzyme concentration which going further than this value has no significant effect on phenol removal. Investigation into time course of phenol removal for both encapsulated and free enzyme showed that encapsulated enzyme had nearly similar efficiency in comparison with the same concentration of free enzyme; however the capsules were reusable up to four cycles without any changes in their efficiency. The ratio of hydrogen peroxide/phenol at which highest phenol removal obtained, found to be dependent on initial phenol concentration and in the solution of 2 and 8 mM phenol it were 1.15 and 0.94 respectively.

Background and the purpose of the study: Date palms (Phoenix dactylifera L., Arecaceae) are one of the oldest cultivated plants which are used in folk medicine for treatment of various diseases. Due to the presence of antioxidant compounds in this plant and the role of oxidative stress in pathogenesis of chronic diseases, the aim of this study was to determine the antioxidant activity and total phenolic contents of date palm fruits extracts with water, methanol 50%, DMSO, and mixture of water-methanol-acetone-formic acid (20:40:40:0.1).Methods: Antioxidant activity of extracts were measured by two tests: inhibition of 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical and FRAP (Ferric Reducing Antioxidant Power).Results and major conclusion: Among 10 different varieties which were examined, the DMSO extract of Khenizi showed the highest antioxidant activity with the FRAP value of 3279.48 mmol/100 g of the dry plant and DPPH inhibitory percentage of 56.61%. DPPH scavenging radical and FRAP values of some varieties including Khenrizi, Sayer, Shahabi and Maktub showed a significant increase and were comparable to a-tocopherol (10 mg/L) when extracted by DMSO. Formic acid extract of Shahabi variety with 276.85 mg GAE/100 g of the dry plant showed the highest total phenolic content compared to other varieties. There was no correlation between accumulation of total phenol and antioxidant activity of extracts, explaining existence of other antioxidant components in date.

Background: Phenols and parabens have been associated with various adverse health outcomes. However, their relationship with breast cancer remains inconsistent, and the combined effect is still unknown. This study aimed to examine the association between mixed phenols and parabens and breast cancer among female adults. Method: Participants for this study were obtained from six cycles of the National Health and Nutrition Examination Survey (NHANES). The weighted logistic regression model was employed to investigate the relationship between individual chemicals and breast cancer. Furthermore, the weighted quantile sum (WQS) regression was used to assess the joint effects of phenols and parabens on breast cancer. Results: The study included 4993 participants, with 154 women diagnosed with breast cancer. After adjusting for all potential covariates, triclosan (TCS) showed a positive association with breast cancer (OR for Q3 = 2.12, 95% CI: 1.23-3.65), while propylparaben (PrPB) exhibited a negative association with breast cancer (OR for Q4 = 0.48, 95% CI: 0.23-0.98). The WQS regression mode found no significant difference between mixed chemicals and breast cancer (OR for positive model = 1.09, 95% CI: 0.65-1.84 and OR for negative model = 0.95, 95% CI: 0.57-1.58). Conclusion: Exposure to phenols and parabens has distinct effects on breast cancer risk. High-quality research is essential to obtain conclusions that are more reliable and uncover potential underlying mechanisms.

The potential of various organisms to metabolize organic compounds has been observed to be a potentially effective means in disposing of hazardous and toxic wastes. Phenols and their compounds have long been recognized as one of the most recalcitrant and persistent organic chemicals in the environment. The bioremediation potential of an indigenous Pseudomonas fluorescence was studied in batch culture using synthetic phenol in water in the concentration range of (100 –500) mg/L as a model limiting substrate. The effect of initial phenol concentration on the degradation process was investigated. Phenol was completely degraded at different cultivation times for the different initial phenol concentrations. Increasing the initial phenol concentration from 100 mg/L to 500 mg/L increased the lag phase from 0 to 66 h and correspondingly prolonged the degradation process from 84 h to 354 h. There was decrease in biodegradation rate as initial phenol concentration increased. Fitting data into Monod kinetic model showed the inhibition effect of phenol The kinetic parameters have been estimated up to initial phenol concentration of 500 mg/L. The rsmax decreased and Ks increased with higher concentration of phenol. The rsmax has been found to be a strong function of initial phenol concentration. The culture followed substrate inhibition kinetics and the specific phenol consumption rates were fitted to Haldane, Yano and Koga, Aiba et al., Teissier and Webb models. Between the five inhibition models, the Haldane model was found to give the best fit. Therefore, the biokinetic constants estimated using these models showed good potential of the Pseudomonas fluorescence and the possibility of using it in bioremediation of phenol waste effluents.