APPLICATION OF MATRIX SOLID-PHASE DISPERSION-HPLC METHOD TO DETERMINE ELLAGIC ACID AND GALLIC ACID IN GALLS OF OAK TREES

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HASHEMI P.; DANESHFAR A.

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Seminar Paper

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Seminar: IRANIAN CHEMISTRY CONGRESS
Year:2013 | Issue:16

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Title

APPLICATION OF MATRIX SOLID-PHASE DISPERSION-HPLC METHOD TO DETERMINE ELLAGIC ACID AND GALLIC ACID IN GALLS OF OAK TREES

Author(s)

HASHEMI P. | DANESHFAR A.

Keywords

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Abstract

GALLS OR CECIDIA ARE OUTGROWTHS ON THE SURFACE OF LIFEFORMS. PLANT GALLS ARE ABNORMAL OUTGROWTHS OF PLANT TISSUES AND CAN BE CAUSED BY VARIOUS PARASITES, FROM FUNGI AND BACTERIA, TO INSECTS AND MITES. GALLS ARE RICH IN RESINS AND TANNIC ACID AND HAVE BEEN USED IN THE MANUFACTURE OF PERMANENT INKS (SUCH AS IRON GALL INK) AND ASTRINGENT OINTMENTS, IN DYEING, AND IN TANNING. MATRIX SOLID-PHASE DISPERSION IS A SAMPLE PREPARATION STRATEGY WIDELY APPLIED TO SOLID, SEMISOLID OR VISCOUS SAMPLES, INCLUDING ANIMAL TISSUES AND FOODS WITH A HIGH LIPIDIC CONTENT. THE PROCESS CONSISTS IN BLENDING THE MATRIX ONTO A SOLID SUPPORT, ALLOWING THE MATRIX CELL DISRUPTION AND THE SUBSEQUENT EXTRACTION OF TARGET ANALYTES BY MEANS OF A SUITABLE ELUTION SOLVENT. SEVERAL TYPES OF GALLE IS INVESTIGATED FOR DETERMINATION ELLAGIC ACID, GALLIC ACID. DIFFERENT MSPD SORBENTS AND VARIOUS ELUTION AGENTS WERE TESTED. IN FINAL CONDITION, 0.01GR OF SAMPLE (GALL) MIXED WITH 0.05 GR OF SILICA-GEL, THEN WERE BLENDED WITH 40ΜL OF DISPERSIVE SOLVENT (CH2CL2) AND THE MSPD COLUMN WAS ELUTED WITH 350ML OF METHANOL. THE EXTRACT WAS THEN ANALAZED BY HPLC. QUANTITATIVE RECOVERIES (>99.5%) WERE OBTAINED AND RSD VALUES WERE LOWER THAN 7% IN ALL CASES.

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APA: Copy

HASHEMI, P., & DANESHFAR, A.. (2013). APPLICATION OF MATRIX SOLID-PHASE DISPERSION-HPLC METHOD TO DETERMINE ELLAGIC ACID AND GALLIC ACID IN GALLS OF OAK TREES. IRANIAN CHEMISTRY CONGRESS. SID. https://sid.ir/paper/914140/en

Vancouver: Copy

HASHEMI P., DANESHFAR A.. APPLICATION OF MATRIX SOLID-PHASE DISPERSION-HPLC METHOD TO DETERMINE ELLAGIC ACID AND GALLIC ACID IN GALLS OF OAK TREES. 2013. Available from: https://sid.ir/paper/914140/en

IEEE: Copy

P. HASHEMI, and A. DANESHFAR, “APPLICATION OF MATRIX SOLID-PHASE DISPERSION-HPLC METHOD TO DETERMINE ELLAGIC ACID AND GALLIC ACID IN GALLS OF OAK TREES,” presented at the IRANIAN CHEMISTRY CONGRESS. 2013, [Online]. Available: https://sid.ir/paper/914140/en

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