Introduction and aim: Iron deficiency anemia is the most common type of anemia. The prevalence of anemia in developing countries is 36% but in developed countries is 8%. 20%-30% of Iranian people are anemic. In this study we attempted to determine prevalence of iron deficiency anemia in Birjand adolescents. Material and methods: in this cross-sectional study, a total of 480 high school students (260 males and 220 females) are selected by clustering sampling and CBC is done for all of them. Iron deficiency was defined as a serum ferreting below level of 12ng/ml, anemia was defined as a hemoglobin level below 12.3mg/dl in girls and below 14 mg/dl in boys and iron deficiency anemia was defined if both of them were below normal. Then, microcytosis cases, after taking oral iron for period of one month were tested again. Results: From among 480 students, 29 (6.5%) suffered from microcytosis. Prevalence of iron deficiency, anemia and iron deficiency anemia in these students was 44.82, 41.73, 24.13. Comparison between mean and SO of hematological markers in male and female student were significant (p=0.000).]n individuals without b thalassemia with microcytosis mean of HCT, MCY, MCH, in one stage of screening were 44.],75, 24.5 and after taking oral iron for one month period increased to 43.4,79.7,25.8. Conclusion: Prevalence of iron deficiency anemia in Birjand school students in comparison with other studies is very high.