Certainty about the water quality for drinking, agricultural, industrial or recreational purposes is of prime importance. Accordingly assessment of river water quality can be an important element in the evaluation of water resources. The present study investigated the water quality of Aras River to provide an overview of the relationship and sources of physicochemical variables. A total of 20 samples were taken from the Aras River at 10 stations of which 10 samples were used for the analysis of heavy metals and 10 samples for nitrate, nitrite, Na, Ca, K, pH, EC and TDS variables. Atomic Absorption Spectro-photometry, Spectrophotometer, Flame Photometer, and pH and EC Meters were used for measuring water quality variables. The results showed that the mean concentration of nitrate, nitrite, sodium, potassium, calcium, and TDS in the Aras River were 297, 0.57, 67.3, 6.4, 57.4, and 1014.73 mg/l, respectively. Lead and cadmium contents were measured as 0.07 and 0.61 mg/l, respectively. Also the mean value of electrical conductivity was 1254.3 μs/cm and the mean value of pH was 8.22. The concentration of nitrate, nitrite and electrical conductivity were higher than the acceptable WHO standard and other variables were less than or within the WHO standard range. It seems that municipal wastewater and agricultural runoff are important factors affecting the water quality in Aras River.