In order to study of genetic variation and relationships among yield and quality traits in alfalfa (Medicago sativa L.), seventy two accessions were studied for yield, morphological and quality traits using a lattice design with 4 replications in Alborz Research Center, Karaj, Iran during 2002 to 2004. For dry matter yield (DMY), the accessions Karaj74, Kurdestanl90, Kazakhistan 2198, Ahar 20253 and France 1008 had higher production. The accessions of Karaj 1003, France 1008, Tabriz 248 and Itlay320 had higher values for both yield and quality traits. The results of path analysis showed that plant height and tiller number had a direct and positive effects and flowering date had an indirect and negative effect on DMY production. Results of principal components analysis accounted for 68% of total variation for first four components. Crude protein, dry matter digestibility, water soluble carbohydrates, acid detergent fibers were recognized as the important traits in the first components. In the second component, plant stand, plant diameter, DMY were the most important traits. The accessions were classified into 7 groups based on Ward's clusters method. Accessions in cluster 1 had higher DMY production, lower quality and early flowering date. Accessions in cluster 2 had lower quality, higher stand and higher insect resistance. Accessions in cluster 3 had medium values for quality, but higher values for DMY, in cluster 4 had higher quality but medium production, in cluster 5 had lower production, higher quality and late flowering date and in cluster6 had early flowering date and medium values for both DMY and quality traits and finally accessions in cluster7 had lower values for both DMY and insect sensitivity.