In this research investigation on parthenolide production and antioxidant defence in callus culture and micropropagated plantlets of feverfew (Tanacetum parthenium (L.) Sch. Bip. Family: Asteraceae) wounding, abscisic acid (ABA) and salicylic acid (SA) treatments were done.For this purpose, seeds were cultured on MS/4 medium with the whole vitamins, and shoot explants of seedlings, with shoot tip and without shoot tip and also, were cultured on MS medium supplemented with NAA (0.54 mM)and BAP (4.44 mM). The growth of plantlets was computed as dry weight.For biochemical investigations, shoot tip explants were cut from two months old plantlets and treated with ABA, SA and wounding (leaves discarded) for 10 hours in MS liquid medium. Parthenolide content was measured by HPLC. Antioxidant defense was evaluated as qualitative peroxidase activity.Callus tissues were obtained from leaf explants of seedlings, in MS medium supplemented with NAA (2mg.L-1) and BAP (0.5mg,L-1).One month old calli were transferred to liquid medium with ABA, SA and DMSO treatments. After one weak, parthenolide content and peroxidase activity were measured as above. Parthenolide content of control plantlets in this investigation was 0.00863±0.00283, but SA+ Wounding treatment in plantlets showed the less parthenolide content and the maximum dry weight. Parthenolide production in treatments was decreased as the growth increased. In callus tissues, parthenolide content was not different significantly between treatments, and approximately half of the plantlets. Qualitative variation in antioxidant defense was shown by electrophoretic patterns and we had an extra band in wounding treatment in micropropagated plantlets. Anionic bands were stronger than cationic bands. Electrophoretic bands had no difference in callus tissues.