Rubella is an acute exanthematous viral infection in both children and adults. The disease may cause severe congenital anomalies in newborn if exposed to this virus during early pregnancy. The objective of this study was to determine seroprevalence of rubella among pre-marriage females living in Ahwaz city. Rubella IgG antibody was measured using ELIZA method. The results showed that among the 400 randomly selected women who were referred for routine per - marriage tests; 366 cases (93%) were seropositive, 28 cases seronegative and the remaining 6 cases were suspicious and therefore were omitted from the study. Based on the result obtained from the worldwide vaccination shows a rate of 95% immunity against rubella. Furthermore, considering that there were insignificant differences in the level of protection between the vaccinated and development of natural immunity; and if the results from similar studies in other parts of the country show same level of protection, it may be concluded that vaccination programme against rubella, at the present time, is not an urgent priority in Iran.