Chemical grouts are developed in response to the need to develop strength and control water flow in geologic units, where the pore sizes in the rock or soil units are too small to allow the introduction of conventional Portland cement suspensions. Due to successful experience in the utilization of chemical grouting over the last five decades, this method has been used for water sealing of part of the karkheh dam foundation, in one of the access galleries of the dam, named galleri 950. Two stages of chemical grout testing have been carried out to obtain some primary objectives of chemical grouting. In stage one of the chemical grout testing, which ended in the first half of Aban 1383, seven triangular holes, with a depth of five meters, were used, which were drilled in a conglomerate trench, near gallery 950. In stage two, which ended in late Esfand 1383, linear holes, with a space of about 1.8m and a depth of 60m, were used. These holes were drilled in gallery 950. The conditions existing at this stage of the chemical grouting were very near to the actual chemical grouting in gallery 950. Chemical grout testing in the Karkheh dam proved the efficiency of the chemical grouting method in seepage control, as well as resulting in valuable and unique experience, including design of a correct program for chemical grouting, mix design, solving execution problems and selection of a proper preparation and grouting system. In this paper, chemical grouting is briefly introduced, then, the results and experiences obtained from chemical grout testing in the Karkheh dam are presented.