Introduction: Nursing is a job based on ethical and legal commitments. one of the most significant responsibilities of every nurse is to know the rules and regulations of his/her job. Methods: This cross-sectional descriptive study was conducted to determine the nursing personnel attitude about their professional rules and responsibilities in jahrom hospitals. The data were collected by a two-part questionnaire. The first part contained demographic data and the second part contained 50 questions, 40 of which were about professional rules and regulations (part one), 7 questions about nursing duties ( part two ) and 3 about committing professional crimes (part 3). The samples involved all of personnel in 2 hospitals (271). Finally 167 questionnaires were analyzed by SPSS software The percent of the answers to each question was also determined.Results: The findings showed that 60% of the participants were from astad Motahari hospital (O.M.H ) and 40% from paymanieh hospital ( P.H ).The Mean ago of the samples in (O.M.H) was 33.5 and in P.H was 29. Also the mean range of job time in O.M.H was 11.5 and in P.H was 7.5. 65.3% of personnel had B.S nursing degree and 23.4% were auxiliary nurses. The mean rank of the questionnaire was 25. The mean rank of the knowledge in 2 hospitals was 37.52. In O.M.H it , was 36.88 and in P.H, 38.48% there was a significant difference between the mean rank of knowledge in P.H (M=29.44 SD±3.14) and in O.M.H (M=28.03 SD±2.29) in part one (p =0/000). However, there were no significant difference between the mean rank of knowledge and age, education, marriage job time and type of responsibility.Assessment of the answers to the questions revealed that the knowledge of 135 (80.8%) personnel about taking oral consent and 95( 56.9% ) about the consent of a patiem. seeking help or nursing care over the phone, and 101( 60.5% ) about finding one of the parents and taking the consent before doing any relatively urgent treatment for their children was poor. Also 133 nurses (79.6%) didn't agree damage to the patients due to the negligenle of health care providers. 72 (43.1%) said that if work problems occurred in their workplaces, they didn't know where and to which coust of law to reffer to and 133 (79.6%) said that if there were some professional problems they wouldn't have been supported by their employers.Conclusion: The results showed that the mean rank of knowledge in three parts was higher than the mean rank of the best but in part one the mean rank of knowledge in P.H was higher than O.M.H. probably one reason is that there are more B.S nursing graduates that work in P.H and pass the nursing ethies course. The knowledge of personnel about some important rules was poor. So the nurses must be educated about some professional rules and the course of nursing law must be added to nursing curriculum. Also by preparing notebooks and in service education, the knowledge of the personnel must be educated.