Mono Fatty Acid Esters (MFAE) in biodiesel fuel (Palmitic, Stearic, Oleic, Linoleic and Linolenic), imposes unique properties that directly affect the combustion process and engine performance. In this study, the effects of each MFAE in biodiesel fuel on diesel engine brake power were studied. Biodiesel fuel, fatty acid ethyl esters from sunflower oil, soybean oil, corn oil, rapeseed oil, waste oil and their blemdings were used in this research. The biodiesel fuels were tested on MT4-244 diesel engine under full load and 2000 rpm. Then, engine brake power was modeled as a non-linear regression function based on percentage of mono fatty acid ethyl ester (MFAEE). Modeling results showed that the saturated fatty acids with short chain hydrocarbons Stearic (C16=0) and Palmitic (C18=0) have the greatest effect on engine power. Unsaturated fatty acids Oleic (C18=1), Linoleic (C18=2) and Linolenic (C18=3) have the least effect on engine power output. Also, Engine power is reduced by increasing the percentage of unsaturated fatty acids in biodiesel fuel. Ethyl ester of Linolenic, which has three unsaturated carbon bond, reduced engine power. Thus, the biodiesel fuel produced from saturated oils, has ability to increase engine brake power.