nowadays, considering the speed of technological progress and increasing attention to innovation as drivers of economic development, Attention to the design and development of innovation ecosystems more than ever is growing. Innovation ecosystems develop and evolve on the subject of technology, sector or industry as a network and strategically in order to create entrepreneurial activities. One of the most important steps on the path of design of innovation ecosystems is search, identify, select and deployment of key actors in the innovation ecosystems. This study is an applied research with qualitative approach using multiple case study that was carried out to identify the key actors in the design of petrochemical downstream industry innovation ecosystem. The findings show that six actors will play key role in the development of petrochemical downstream industry innovation ecosystem that include orchestrator, Accelerators, Human and physical infrastructure suppliers, venture capital, lead users and lead experts, Which respectively are business development holding as a orchestrator, NECO industry management as an accelerator, Iran Polymer and Petrochemical Institute, Petrochemical Research and Technology Company (NPC-RT), research institute of petroleum industry, Chemistry & Chemical Engineering Research Center of Iran, and Department of Chemical Engineering in Sharif, Amir Kabir and Tehran universities as suppliers of Human and physical infrastructures, Innovation and Development Fund as a venture capital, public relations unit of National Petrochemical Company and Companies with demo plant as lead users, knowledge based companies as lead experts in Iranian petrochemical downstream industry innovation ecosystem.