Six Holstein cows (120±20 d in milk) were used in a 3x3 replicated Latin square design to investigate the effect of different particle sizes of ground barley grain on digestibility, degradation rate and lactation performance. Geometric mean diameters of the barley particles were 0.94, 1.93 and 2.90 mm for treatments 1, 2 and 3, respectively. Diets were only different in barley particle size and all cows received diets containing 40% corn silage and 60% concentrate (DM basis) composed of 50% ground barley. The differences among dry matter intake (DMI), milk fat percentage, milk total solid percentage, daily fat yield, dry matter digestibility, urinary and ruminal pH, daily body weight change, and fecal particle size distribution were not significant. Treatment 3 caused a decrease (P<0.05) in milk protein percentage, daily milk yield and fecal pH compared to treatments 1 and 2; no other treatment differences were significant. With increasing barley particle size, fecal dry matter was increased but daily milk protein yield was decreased (P<0.05). Significant differences (P<0.05) in 4% FCM, 4% FCM/DMI daily, milk lactose yield, daily total solids yield and organic matter digestibility were observed between treatments 1 and 3. No other treatment effects were observed significant. The soluble fraction, the potential degradable fraction, ruminal degradation rate and the effective degradability of dry matter increased linearly for treatments 1, 2 and 3, respectively. It is concluded that fine grinding of barley (0.94 mm) which is commonly used on dairy farms improved OM digestibility, milk yield, milk protein percentage and production and would be recommended for feeding conditions similar to those of the present experiment.