Aims: Warriors' education and complete domination over self-aid and buddy-aid is one the most effective approaches for enhancing their safety in military operations. The present study has been performed to "investigate the effect of self-aid and buddy-aid education using lecture and multimedia software package on performance level of military personnel".Methods: In this semi-experimental study in 2010, sixty subjects from the selected military battalion of Tehran and Mashhad were investigated. Participants were randomly allocated into two groups of thirty each and were educated using lecture and multimedia software package. In the end, they were evaluated in terms of performance level through objective structured clinical examination. Data were analyzed by SPSS15 statistical software and nonparametric statistical tests.Results: At "vital signs monitoring, " "carrying method of injured, " "head bandage, " "self-care and decontamination of biological attacks, " stations, no significant difference was observed between the two groups (p>0.05), while, in "cardiac and pulmonary rehabilitation, " "first-aid measures for eye burns, " and "splinting" stations, the results indicated a statistical difference by Man-Whitney (p<0.001). In "bleeding monitoring" station, lecture-trained group showed more acceptable results compared to software-educated group (p<0.05).Conclusion: Regarding more effectiveness of multimedia software in some stations, especially CPR, and considering lecture-related limitations, expanding multimedia software application is recommended in self-aid and buddy-aid education.