During 1997- 99 one seed samples of each 17 sesame cultivars (totally 17 samples) were collected from 10 localities in Iran. Blotter method and culture media (PDA, PMA, WA, CA, SNA, CLA, CYA, KCLA, WALM, MEA) were used to isolate and identify the mycoflora present on different parts of seeds. Totally, 145 isolates were obtained which included 34 species of 15 genera. The identified fungi were: 1.Acremonium strictum, 2.Alternaria alternata, 3. A. raphani, 4. A. sesami, 5. Alternaria sp., 6. Aspergillus flavus, 7. A. niger, 8. A. ochraceus, 9. A. te1TeUS, 10. Chaetomium elatum, 11. C. funicolum, 12. C. olivaceum, 13. Cladosporium cladosporioides, 14. C. elatum, 15. C. herbarum, 16. C. macroCalpum, 17. C. oxysporum, 18. Fusarium moniliforme, 19. F. oxysporum, 20. Paecilomyces variotii, 21. Paecilomyces sp., 22. Penicillium brevicompactum, 23. P. chrysogenum, 24. P. citrinum, 25. P. digitatum, 26. Rhizopus orysae, 27. Scopulariopsis brevicaulis, 28. Stachybotry chartarum, 29. Stemphylium botryosum, 30. Tiarosporella phaseolin a, 31. Trichoderma harzianum, 32. Ulocladium atrum, 33. U. lanuginosum and 34. Ulocladium sp.
The fungi indicated by Nos. 1, 3, 12, 16, 17, 27, 33 were new for Iran mycoflora, Nos. 1, 3, 5, 10, 11, 12, 13, 14, 15, 16, 17, 20, 21, 22, 23, 25, 26, 27, 28, 29, 31, 32, 33, 34 were reported from sesame seeds for the first time, and Nos., 2, 3, 6, 7 and 26 were dominant.