Bio-climate studies, from the viewpoint of human comfort is the foundation of management planning. In the present study of four climatic parameters including apparent temperature, Standard Effective Temperature, Thermo hygrometric and wind for the study of human comfort was used in 14 cities. Areas of study include Abadan, Ahvaz, Bandar Lengeh and Bandar Abbas, Bushehr, Dezful, Esfahan, Kerman, Sabzevar, Semnan, Zahedan, Kashan, Yazd and Zabol, which were classified in two summer climates of very hot and hot. Meteorological variables used included daily values of air temperature, dew point temperature, relative humidity and wind speed at a height of two meters of summer, in the period 1385-1350. Results showed that relative humidity as a factor in comparison with daily dew point temperature as a factor at night represents the human comfort level in most coastal areas and is non-coastal. Thus, the standard effective temperature index (SET) because of a combination of weathering study areas daily realities, is more compliant. Accordingly, Bio-climate spectrum study areas, was in the quarter from Bio-climate sultry summer, with a range 27.5-30, Bio-climate to cool, with a range 15.5-17.8. Overall index based SET, in June that the weather warming began, eight cities in descriptive categories were hot and very hot. In August, compared with urban warming July, the city increased to nine, and in September with completion in summer, the number of cities to the city six decreased. Also, the SET index was based on any of the cities and none of the months of summer heat stress and severe cold stress. In the cities of Bandar Abbas, Bushehr and Bandar Lengeh, sultry heat stress is due to close proximity to the Persian Gulf, seems logical.