This research was carried out for studying the effect of different plant densities and split application of nitrogen fertilizer on quantitative yield of Brussels sprout Ula De La Halle cultivar in Miyaneh region, Iran. Treatments were arranged as split plot based on randomized complete block design with three replications in 2008. Plant densities of 1.38, 1.58 and 1.82 plant/m² and split application of necessary nitrogen fertilizer including whole nitrogen fertilizer application in transplanting stage and splitting it, half in transplanting stage and the rest one month afterward, were considered as the main and sub-plots, respectively. The results revealed that the interaction between plant density and nitrogen splitting was significant only in stem diameter trait. Plant density influenced plant height, stem diameter, leaf number, petiole length, petiole diameter, petiole weight, plant width, sprout number and sprout yield, significantly. Stem diameter, petiole diameter and plant width were decreased by increasing plant density. There was a positive correlation between the plant density and shrub height, leaf number, petiole length, petiole weight, sprout number and sprout yield. Nitrogen fertilizer splitting influenced plant height, stem diameter, petiole diameter and petiole weight, significantly. The application of the whole nitrogen fertilizer increased the plant height and stem diameter.