To evaluate the variation and deterioration of accessions of Dactylis glomerata seeds, which have been collected in Natural Resources Gene Bank, research programs were carried out using 31 collected accessions. All of accessions were independently examined in complete randomized design with three replications in two different situations, named as germinator and glasshouse conditions. The test in germinator condition included, Germination Percentage (G.P), speed of germination and vigor and the test in glasshouse, included G.P, speed of germination, plant height, number of tillers and root length in 40 days after planting. Significant difference was found among accessions examined in this experiment. Variation between accessions in measured characters was found to be significant in one or both of environments. The results of correlation coefficient study were shown that, 1000 seed weight could affect G.P and preliminary growth of plants in both environments. It was found that speed of germination, could be very good indicator of seed vigor. This result was endorsed by regression model. Negative correlation was found between reduction of G.P, speed of germination and seed vigor in germinator condition and G.P, speed of germination and plant height in glasshouse. Therefore, seed deterioration not only could cause reduction of G.P, but also is able to decrease the speed of germination and seed vigor. In all of the Dactylis glomerata accessions, reduction of G.P, highly affected by origin of accessions. It was not possible to differentiate between the effect of maintaining condition and origin of seed collection. Therefore, it suggests that, for better managing the Gene Bank, accessions, but not species, have to be considered as regeneration unites of collected seeds.