In order to investigate the effect of different levels of salinity stress on Anions and Cations absorption concentration in different lines and cultivars of rapeseed (Brassica napus L.), a factorial experiment in completely randomized design with three replications was carried out at research greenhouse in Agricultural Research center of Mazandaran in 2009-2010 cropping year. Four levels of water salinity including (0 (control), 4, 8 and 12 ds/m-1) were considered as the first factor and seven cultivars including (Zarfam, Sarigol, Line 14 Line 18, Line 111, RG003 and Hayola 401) as the second factor. The results showed the most absorption concentrations of Mg, Ca and Na was obtained for RG003 cultivars and Line 14, respectively and also the highest amount of K and Cl absorption concentrations were belonged to RG003, Zarfam and Sarigol cultivars in vegetative stage. As the highest amount of Line 14 and RG003 cultivars was obtained in Ca and Na concentrations, the highest amount of Na, K and Cl were related to Line 111, Sarigol, Zarfam and RG003, respectively at reproductive stage. In addition, due to increasing salinity stress in elements concentration in comparison with vegetative stage had less variation, as in generative stage, absorption concentration of Na and Cl elements were about eight and two times of their amounts in comparison with control treatments, respectively.