This study was conducted in order to consider the effect of water soluble fraction of crude oil on the accumulation of heavy metals (Ni, Pb, Cd & V) in muscle tissue of Pelates quadrilineatus from the Persian Gulf. The study was carried out in spring 2006 in the Ecology Research Center of the Persian Gulf. Two solutions with 0ppm (Control) and 12 ppm dosages of crude oil were prepared. Then 24 fish from this species that were caught from 6 stations in the northern part of the Persian Gulf were exposed to 12 ppm dosage of crude oil for 8 days in long-term bioassay examination. After 8 days exposed fish were freezed and packed seprately and sent to the laboratory for determination of heavy metals accumulated in their muscle. Heavy metals concenteration measurment was done with Atomic Absorbtion model Philips PU 3400. The result of data analysis shows that the mean (x¯±STD) accumulation amounts of V, Ni, Cd & Pb in tissues of control fish were 0.15±0.02 ppm, 0.36±0.03ppm, 0.02±0.01 ppm and 1.1±0.04 ppm respectively. The mean (x¯±STD) accumulation amounts of V, Ni, Cd & Pb in tissues of fish which were exposed to 12 ppm dosage of crude oil were 0.25±0.04 ppm, 0.57±0.03 ppm, 0.09±0.05 ppm and 1.57±0.11 ppm respectively. According to T-test we concluded that there is a significant difference (p< 0.05) between two mentioned dosages of crude oil in muscle tissues of this fish. Also in the fish of both treatments the order of heavy metals accumulation was Pb> Ni> V> Cd that was significant according to T-test experiment. On the other hand, the high amount of heavy metals in the control fish is due to pollution of Persian Gulf. Also, the mean concentrations of Pb, V & Cd in muscle tissue of control fish were below the international guidelines for human consumption.