In this study, fatty acid composition of following two aquatic insects: Limnephilus sp. and Hydropsyche sp, were studied during two season (Spring and Summer 2010) in the Doab Alashtar river in the lorestan Province.According to the results, Hydropsyche sp. with average of 31.72% (of total fatty acid) MUFA, had higher level of MUFA in comparison with Limnephilus sp. with average of 30.03% (of total fatty acid) (p<0.05). The average level of PUFA in Hydropsyche sp. and Limnephilus sp. were recorded 31.94 % and 24.84%, respectively.According to the result, levels of PUFA in Hydropsyche sp. and Limnephilus sp. were measured 31.94% and 24.84% (of total fatty acid), respectively and levels of SFA in Hydropsche sp. and Limnephilus sp. were recorded 30.59 % and 29.69% (of total fatty acid), respectively. The result showed that PUFA in Hydropsycche sp. and MUFA in Limnephilus sp., were dominant fatty acid. According to the result following fatty acids: 18: 3n-3 (13.19 percent of total fatty acid), 18: 2n-6 (8.2 % of fatty acid) and 20: 4n-6 (4.69 % of fatty acid) were more abundant fatty acids in Hydropsyche sp., respectively and in Limnephilus sp., following fatty acids: 18: 2n-6 (11.5% of total fatty acid), 18: 3n-3 (9.6% of total fatty acid) and 20: 5n-3 (2.9 % of total fatty acid) were more abundant fatty acids in this species respectively. Among MUFA, 18: 1n-9 was the most abundant with following levels of 20.89 % and 19.17% (of total fatty acid) in Limnephilus sp. and Hydropsyche sp., respectively. Among SFA, 16: 0 was the most abundant with following levels of 26.31% and 21.15% (of total fatty acid) in Limnephilus sp. and Hydropsyche sp., respectively. According to the results, the level of following fatty acids: 14: 0, 14: 1n-5, 20: 0, 22: 0, 20: 3n-3, DHA/EPA and n3/n6 had significant differences between spring and summer (p<0.05) but levels of PUFA, MUFA, DHA, n6 and FAME showed no significant differences according to the two studied season (p³0.05). The level of following fatty acids: SFA, PUFA, n3, n6, DHA, ARA, n3/n6 and MUFA had significant differences according to genius (p<0.05) but levels of EPA, DHA/EPA and n3/n6 had no significant differences according to the two studied genius (p³0.05).