In order to determine the optimum sowing date of different medicinal flax genotypes, a split plot experiment with completely randomized block design with 12 treatments and 4 replications were conducted at experimental farm of Yasooj Islamic Azad University. The treatments were composed of four different combinations of sowing dates ((Feb. 20, Mar. 6, Mar. 21, and Apr. 4) and 3 flax cultivars (Somaco, India, and Foster). The studied traits were as follows: plant height, 1000-kernel weight, harvest index, grain yield, Biological yield, Leaf Area Index, Crop Growth Rate and Relative Growth Rate. The results showed that there is a significant effect of between various sowing dates regarding the plant height, biological yield, Relative Growth Rate, Net Photosynthesis Rate, Leaf Area Index, grain yield, and 1000-kernel weight and for harvest index. Besides, the analysis of the data variance shows that there is a significant effect of between investigated genotypes regarding the plant height, biological yield, Crop Growth Rate, Relative Growth Rate, Leaf Area Index, grain yield. According to the results of experiment, the first sowing date (Feb. 20) and Somaco cultivar are recommended as the suitable for higher grain yield.