This study was conducted to evaluate 13 new triticale genotypes based on yield and nutritive value traits using RCBD with three replications at Seed and Plant Improvement Institute Karaj, Iran in 2008-2009 cropping seasons. Analysis of variance indicated significant differences (p<0.01) for grain filling period, thousand kernel weight, number of seed per spike, biological yield, chlorophyl content and plant height. Chemical composition (Dry matter, crude protein, crude fiber, ash, crude fat, calcium, phosphorus and nitrogen-free extract) according to standard methods (AOAC, 2000), sugar and total starch with chemical method, some anti-nutritional substances of 13 lines of triticale was measured. The results showed that the average and range changes values of anti-nutritional compounds in the thirteen lines of triticale, total b-glucan, soluble b-glucan, insoluble b-glucan, total arabinoxylane, soluble fiber, insoluble fiber and total soluble and insoluble fiber were respectively 0.950, 0.018, 0.931, 3.98, 1.14, 18.75 and 19.89 percent. The measurement of anti-nutritional compounds (Total b-glucan and total arabinoxylane), showed that the total amount of arabinoxylane obtained more than the total amount of b-glucan so the arabinoxylane is the most important and most anti-nutritional compoundsl in triticale. According to the final results the experimental genotype no. 5 (ET-82-15) had highest grain yield and lowest Arabinoxylane so selected for Onfarm trials.