VETERINARY CLINICAL PATHOLOGY (VETERINARY JOURNAL TABRIZ)
Year:2020 | Volume:13 | Issue:4 (52) |Papers Count:7

Infections with gastrointestinal (GI) nematodes severely affect small ruminant health and compromise their productivity and reproductive performances and can be a major cause of economic losses in small ruminant production. High burden of infections with nematodes may lead to death; and under field conditions, most infections are usually mixed consisting of different species of nematodes. Sheep and goats are infected with gastrointestinal nematodes while grazing and the biology of the infective larvae on the pastures is important in planning effective control programs. In general, the development of the free-living stages of gastrointestinal nematodes is largely dependent on temperature, with development rate increasing at warmer temperatures, while moisture is also required for the development cycle to proceed. Temperature also influences grass growth and hence exposure independently of larval development and survival. East Azerbaijan province in terms of livestock animal population in particular sheep breeding is one of the most important provinces of Iran and due to the amount of rainfall is susceptible to worm’ s contamination in livestock animal. In this survey, fecal samples of 5934 sheep in 19 cities in East Azerbaijan, from 2002-2009 were examined by the Clayton-Lane method. Briefly, a sample of feces, 1-5 g, was well mixed with water (about 30-50 ml) and strained through a sieve (1 mm mesh) to remove coarse fecal material. The mixture was sedimented for 10-15 minutes on the bench, or by light centrifugation for two or three occasions, until the supernatant was clear. The sediment was then mixed with a saturated solution of sugar in a centrifuge tube (15 ml volume) and centrifuged for one or two minutes at 500 g. The egg floated and coverslips were placed on the tubes in centrifuge and after 5 min the samples were examined for the presence of eggs at 100x and 400x magnifications under the light microscope and the number eggs of Nematodirus, Marshallagia, Trichuris, unknown nematodes parasite eggs and the whole of the nematode eggs were determined in these cities by their shape and size. These data were analyzed by the SAS software. The results showed that the Kaleibar was the most infested city and the Malekan had the lowest contamination to gastrointestinal nematode parasites. Marshallagia and Trichuris had the maximum and minimum quantities of means with 11. 12± 32. 02 and 2. 23± 13. 78, respectively. A study on the correlation between humidity in the second month and humidity average and fecal egg count showed that there is a positive and significant (p<0. 01) correlation between them. Base on the results of this survey high humidity in regions and seasons are risk factors for propagating worm infestations and distinct handlings are necessary for preventing the mentioned phenomenon. This study survey on geographical situation in 19 cities of East-Azerbaijan indicated that the infestation rate to gastrointestinal nematodes in south of province (Malkan city) is at the minimum rate and in north province (Kaleibar city) is in maximum rate. Because the north region of East-Azerbaijan province is warmer and moister from the other regions, the high rate of infestation to GI nematodes was expected. Furthermore, differences in the population of livestock animals in the two regions are important in the rate of infestation to nematodes. In conclusion, the results of the study denoted that even approve the anthelminthic drugs in the livestock animals, the rate of infestation to GI nematodes is high. Also, the results of this survey are showed that a positive correlation is between EPG and the mean of humidity and this is important that treatment and prophylaxis are needed to decrease the risk of the disease. This research serves as a baseline for future studies on prognosis and prophylaxis of GI nematodes. These regional differences appeared to be systematic and can be ascribed to differences in applied pasture management and climatic conditions, and cause a need for regionally adapted monitoring and control systems.

Materials and methods: To evaluate the Met deficiency as a vital amino acid on histology of small intestine of Japanese quail, 20 male one-day old quails were randomly allocated into 2 groups of 10 birds. One group received Met deficient diet while another group of birds were kept as control with standard diet for 6 weeks. At the end of the experiment, all animals sacrificed by cervical dislocation. Whole length small intestine was removed immediately and immersed in 10% buffered formalin. After fixation, 1cm-thick samples were taken from the middle parts of duodenum (from the gizzard to pancreatic and bile duct), jejunum (from the bile duct to Meckel’ s diverticulum) and ileum (from the Meckel’ s diverticulum to ileo-cecal-colonic junction). After routine histological laboratory methods, 6μ m-thick transverse cross-sections were made, a total number of 10 sections used from each intestinal segment of each bird; sections stained with Haematoxylin and Eosin and Periodic acid-Schiff. For measuring length and width of villi, depth of crypts and goblet cells number Axio vixion Rel 4. 8 software were used. Data were expressed as Mean± SD. Data analysis was performed by Independent-Samples T Test method and differences considered statistically significant at p

The present study was performed to evaluate the effect of adding different levels of EDTA and propylene glycol on quality and survival of Ghezel ram frozen semen. After primary evaluations on fresh semen for parameters including total motility, progressive motility, non-progressive motility, viability, abnormal sperm, and acrosome integrity in total cell and acrosome integrity in dead cells, samples with a concentration of 2. 5 billion sperm and a progressive motility of over 70% and a volume greater than 0. 5 ml were diluted with 1, 1. 5, 2, 2. 5 mM EDTA and 1, 2, 3% propylene glycol as an additive and propylene glycol 7% as a constitute. Straws (250 µ l) were filled with dilution and were placed in a refrigerator for 1. 5 to 2 hours to cool to reach 5° C. After cooling they are placed in 4-5 cm above nitrogen for 8-10 minutes and ultimately were immersed in liquid nitrogen. qualitative parameters of sperm included viability, total motility, progressive and non-progressive motility, hypo osmotic swelling test, percentage of acrosome integrity and acrosome health in dead cells were investigated in 0, 20, 40 and 60 days of freezing processes. The results of this experiment showed that diluent with 1% propylene glycol concentration in comparison with other group improved total and progressive motility, percentage of viability, acrosome integrity and HOST (p0. 05). The results showed that dilutions of EDTA and propylene glycol could improve quantitative parameters of ram semen.

Background and Purpose: Corneal ulcer is one of the most common eye diseases, which can be caused by trauma, chemical agents, and some bacteria, viruses, and chlamydia. Treatment of corneal ulcer has been always a concern in ophthalmology. This concern is due to the major role of cornea in providing and preserving normal vision. Alkali-induced corneal ulcer creates an intense inflammatory reaction to traumatic injuries and this intense inflammation can inhibit the natural epithelial growth and cause fibrosis or scar on cornea. Cornea is an organ in the eye that creates a smooth and clear surface and so, it provides the possibility of light regularly passing through the eye. Any injury of this layer eliminates its transparency and protective capacity. Cornea has few cells and no vessels. Various bacterial, viral, parasitic, and fungal agents can cause severe infections in cornea. After the occurrence of corneal injury, the cells surrounding the injured area including epithelial, stromal, and endothelial cells try to improve the injury by cell division and calling the immune system cells such as neutrophils, macrophages, and monocytes; as a result of this process, the injured area becomes inflamed and edematous. In most cases, due to basic membrane decay and leakage of proteinase, epithelial cells will not be able to provide the connections required for retrieving the layers. So, the distance between epithelial cells increase and the bond between them become weak. As a result of corneal ulcer, increased activity of destructive proteinases and decreased activity of proteases leads to rapid detachment of collagen and other extracellular components of corneal matrix. Significant increase of reactive oxygen species (ROS), increased oxidative condition, and decreased components of antioxidant system occur following corneal injury. In histological studies, various cells of immune system including neutrophils, lymphocytes, monocytes, and macrophages are found in cornea. Increased reactive oxygen species (ROS), increased oxidative stress, and decreased components of antioxidant system are observed in pathologic conditions and in the case of corneal injury. Various studies have reported the role of antioxidants in prevention and treatment of corneal ulcer. The recent studies have indicated that folic acid restores decreased enzymatic and non-enzymatic antioxidants. also, its improving effect is due to inhibition of reactive oxygen species (ROS).

Today, the most important factor in the occurrence of metabolic and reproductive diseases in dairy cattle is the disturbance of natural process in body’ s cellular-molecular events that occur after increasing of oxidants and the formation of oxidative stress. The aim of this study was to evaluate the possibility of a significant association between the serum concentrations of malondialdehyde (MDA) and total antioxidant capacity (TAC), as oxidative stress indexes, with the incidence of subclinical ketosis and clinical endometritis in dairy cows. For this purpose, 101 Holstein dairy cows were divided into 4 groups (healthy, subclinical ketosis, clinical endometritis, and both of them subclinical ketosis-clinical endometritis), and serum levels of MDA and TAC were measured in one month before delivery. The lowest serum concentration of TAC and the highest plasma MDA levels were observed in cows with both of diseases, while control group (healthy cows) had the highest serum concentration of TAC and lowest MDA levels (P≤ 0. 05). There was no significant difference in serum concentrations of TAC and MDA between clinical endometritis and subclinical ketosis groups (p>0. 05). The results of this study demonstrated a significant correlation between serum concentrations MDA and TAC with the incidence of clinical endometritis and prevalence of ketosis-endometritis in dairy cows (P≤ 0. 05).

The ruminant infant is susceptible to neonatal infections due to the immature immune system. Antioxidants can enhance the function of the immune system cells. Garlic is an antioxidant that has been proposed as a prophylactic antibiotic in ruminant infants. The aim of this study was to determine the effect of adding aqueous extract of garlic to milk on the plasma oxidant-antioxidant balance in neonatal goat kids. This study was performed on 30 male and female suckling 7-day-old neonatal goat kids of mixed breed (mean weight 3 kg). The goat kids were divided into 3 equal groups. Each group received one of the following three oral treatments (inclusive 30 ml of saline, 400 IU of vitamin E, 83 mg / kg of aqueous extract of garlic) at every 3 milk meals per 24 hours. At 0 min and 1, 2/5, 5, 12, 18, and 24 hours after the beginning of the first treatment, blood samples were taken and the plasma was discarded and the total antioxidant and total oxidation capacity of the plasma and its oxidative stress index were determined. Compared to negative control, treatment with vitamin E caused a significant tendency of the oxidant-antioxidant balance of the plasma towards the antioxidant (p

Introduction: Nowadays one of the problems of countries around the world, is the use of drugs, including Crack. Crack, sometimes called rock, is a stimulant derived from cocaine treatment, but in Iran, it is derived from heroin derivatives, and consumption of this type of drug can add up to 3-times. Crack users are at an increased risk of both physical and mental illness and social harm. There is also evidence that they present more psychiatric problems than the general population, in addition to the fact that Crack use and mental disorders are mutually aggravated. The aim of this study was to evaluate the effect of a single dose of Crack for the antioxidant system of swelling in Rats. Material and Methods: Thirty Wistar Rats were selected with a mean weight of 25 ± 250 and kept in 5 groups of 6 in a glass aquarium with 12 hours of light and 12 hours of darkness and the same nutritional conditions and free access to water. After acclimation to the Crack medium at a dose of 7. 8 mg/kg, it was injected intraperitoneally (IP) in 4 treatment groups. In the control group, only physiological serum was used. The first group received 3 hours, the second group 6 hours, the third group 24 hours and the fourth group 1 week after the injection of the tail vein after anesthesia. In the control group, blood samples were taken on the first day. One sample was used anticoagulant. Then activity of Blood’ s Catalase, Glutathione Peroxidase and Super-Oxide Dismutase were measured.