Background and objective: Influenza virus H9N2 is a low pathogenic avian influenza that its first outbreak in Iran was reported in 1998 and caused appreciable economic losses in the poultry industry. Vaccines are one of applicable approach for protect the avians from avian influenza viruses. This study aimed to evaluate a killed avian influenza vaccine (produced in Razi vaccine and serum research institute) against current isolates in Shiraz.Materials and Methods: In this study, fifty broiler chickens were divided into 5 ten-bird groups including test and control groups. Forty broiler chickens (group 1, 2, 3 and 4) were vaccinated with the killed oil-emulsion influenza vaccine that was obtained from Razi Vaccine and Serum Research Institute. Ten chickens were used as control group (Nonvaccinated group). After two weeks both vaccinated and control chickens were inoculated with H9N2 influenza viruses that were isolated from chickens farms of Shiraz in 2010. The cloacal and tracheal swab samples were collected from chickens at 1, 3, 5, 7, 9, 11 days after infection and were processed for virus isolation.Results: The results showed that after five days for group 1 and after seven days for groups 2, 3 and 4, virus shedding into tracheal and cloacal samples was significantly decreased. However, the shedding continued for 11 days in control group.Conclusion: The results suggested that the killed oil-emulsion influenza vaccine could efficiently decrease AI replication and its shedding in the broiler chickens.