Background and Aim: This study aimed to compare the level of pain, wound healing, facial edema, and surgeon’ s comfort in surgical extraction of impacted third molars using surgical scalpel versus radiofrequency (RF) incision. Materials and Methods: IThis split-mouth clinical trial evaluated 41 patients with bilateral impacted third molars in one jaw with the same Pederson difficulty index (between 5 and 7, moderate difficulty). The surgical incision was made using a surgical scalpel on one random side and an RF device on the contralateral side. The level of pain was measured using a numerical rating scale (NRS) 7 days postoperatively. The wound healing was evaluated using the wound evaluation scale (WES) 4 weeks postoperatively. Facial edema was quantified using a tape measure 7 days postoperatively. Surgeon’ s comfort was assessed by asking the surgeons regarding the level of easiness of the procedure. The pain score, wound healing score, facial edema, and surgeon’ s comfort in surgical extraction of impacted third molars were compared between the two sides using SPSS 22 via paired t-test and McNemar’ s test. Result: The surgeon’ s comfort was significantly higher in the use of a surgical scalpel (P<0. 001). The difference in pain score (P=0. 95), wound healing (P=0. 32), and facial edema (P>0. 05) was not significant between the two groups. Conclusion: The results of this study showed no significant difference in surgical extraction of impacted third molars using a surgical scalpel or an RF device regarding the level of pain, wound healing, or facial edema.