Background and Aim: Nurses’ psychological wellbeing is important determiner in their professional health and it is necessary for effective caring of patients. The present study aimed at describing the role of job burnout, socioeconomic factors, and working environmental conditions in nurses’ psychological well-being.Materials and Methods: In this cross-sectional and descriptive study, 345 nurses were selected, through convenience sampling, from the hospitals of Isfahan University of Medical Sciences between March and July 2011. They completed Personal Well-being Scale, Burnout Clinical Subtype Questionnaire, Practice Environment Scale of Nursing Index, and Socioeconomic and Occupational Factors questionnaire. The degree of association between different variables was assessed using correlative and explanatory factors of nursing psychological well-being by means of stepwise multiple regression.Results: There was a significantly positive correlation between frenetic activity, working environmental conditions, age, work hours per week, and income with psychological well-being but there was a significantly negative correlation between lack of job challenge, feeling burn out, and type of employment with psychological well-being (P<0.001). Job burn- out, working environmental conditions, income, frenetic activity, and work hours per week were significant precursors of psychological well-being of nurses (P<0.001).Conclusion: Burn-out and worn-outness, inappropriate working environmental conditions and extended long work hours per week, frenetic activity, and low income reduce nurses’ psychological well-being. The findings emphasize on the importance of more effective intervention in burn-out syndrome and inappropriate job conditions to promote their psychological well-being.