Purpose The aim of the present study was to investigate the effects of short-term vitamin D supplementation on the pulmonary function and physical fitness factors in adolescent wrestlers. Methods 36 wrestlers (age: 16. 18 ± 0. 81 years, height: 171. 5 ± 5. 7 cm, weight64. 2± . 36 kg, BMI: 21. 6 ± 1. 75kg/m2, Ft mass: 8. 9± 1. 82%) participated in this study. The subjects in the experimental group received 2000 IU of vitamin D daily and the control group received the same amount of placebo for 14 days, during which time they also performed wrestling training (5 sessions per week). To investigate the research variables, serum 25(OH)D levels, pulmonary function by spirometry test and physical fitness indices were measured twice, before and after supplementation periods. The data were analyzed using the analysis of covariance. Results Results showed that vitamin D levels (23%) and FVC indices (20%), FEV1 (17%), MVV (12%), FEV1 / FVC (11%), FEF25 / 75 (13%) were higher significantly in vitamin D compare with placebo group (P>0. 05). But there was no significant difference between PEF, FEF25, FEF75, FEF50 and physical fitness factors (shuttle run, 50m sprint, sit-ups, pull-ups, push-ups and Sargent jump) in the experimental group compared to the placebo group (P>0. 05). Conclusion The present findings of this study indicated that short-term vitamin D supplementation may improve pulmonary function in adolescent wrestlers by affecting respiratory muscle strength and decreasing airway resistance but did not affect their physical fitness.