This study has been performed to compare DAT1, a new mid-season apple genotype, with summer apple cultivars including ‘ Gala’ , ‘ Golabe-Kohanz’ , ‘ Shafi Abadi’ , and ‘ Golab-e-Isfahan’ grafted on M9, MM106, and MM111 during two consecutive years in Kamalshahr Research Station. The experiments use a factorial design, arranged in a Random Complete Block Design with 3 replicates. Results show that the impacts of cultivars, rootstocks, and their interactions has been significant (P≤ 0. 01) on growth characteristics, having the highest values in ‘ Golab-e-Kohanz’ and ‘ Golab-e-Isfahan’ grafted on MM111, with 92 and 91. 7 cm respectively, while the lowest value occurs in DAT1, grafted on M9 with 53. 7 cm. the highest yield is found in ‘ Golab-e-Isfahan’ , ‘ Golab-e-Kohanz’ , and DAT1 grafted on MM106 and MM111 with 22. 50, 20. 05, and 18. 4 kg, respectively. The largest fruits belong to DAT1, being 211. 80 gr, and the smallest ones are grown on ‘ Golabe-Isfahan’ and ‘ Golab-e-Kohanz’ with 75. 50 and 69. 55 gr, respectively. Regarding flesh firmness, the hardest and softest fruits turn out to be ‘ Gala’ grafted on M9 and DAT1 on MM106, being 5. 8 Kg/cm2 and 3. 7 Kg/cm2, respectively. The maximum soluble solids (18. 5%) has been ‘ Gala’ grafted on all three rootstocks and the lowest one (11. 5%), ‘ Golab-e-Isfahan’ grafted on MM111. The maximum acidity of fruit juice has been found in DAT1 grafted on MM106 with 0. 68% and the lowest one in ‘ Golab-e-Isfahan’ grafted on MM106 with 022%.