Aims and Background: Aims and Background: With respect to the importance of assessing chronic pain acceptance in the elderly, access to a presice instrument for this purpose is essential. Hence, the current study aimed to develop and evaluate a chronic pain acceptance instrument in the elderly.Materials and methods: This study is a mixed method research, implemented in three stages. The grounded theory approach was applied for concept exploration of chronic pain acceptance in the first stage. It was based on the viewpoints of 30 elderly people with chronic pain, 3 relatives and 29 health care providers who participated with purposeful and theoretical sampling methods. In the second stage, instrument sentences were extracted and developed by qualitative part results and review of the valid and related literature, respectively. Psychometric evaluation of the instrument in the third stage was based on face, content, and construct validities, internal consistency and stability reliabilities.Findings: The concept of Chronic pain acceptance was explored in the first stage. Based on participants’ viewpoints in the qualitative part of the study, pain acceptance was to believe in pain, and its related limitations and disabilitites in order to controll it. An elementary instrument with 38 sentencess was developed in the second stage. During the third phase of the study, face and content validities by quality and quantity methods (Content validity ratio average = 0.94, and content validity index average= 0.92) were performed, respectively. Moreover, construct validity was assessed by exploratory factor analysis, and instrument sentences were decreased to 7 item, in which two factors were obtained: the first factor (awareness of pain) including two items and the second one (trying to decrease pain) including 5 items. Finaly, internal consistency (a =0.83) and stability (0.85) were determined for reliability evaluation by retest method.Conclusions: Regarding the precise development process, suitable validity, and reliability of chronic pain acceptance assessment instrument, its application is suggested for pain acceptance assessment and effective pain management in the elderly.