Effects of trifluralin (2, 6-dinitro-N, N-dipropyl-4-(trifluoromethyl) benzenamine) and iron on morphological characteristics and iron absorption of five cultivars of soybeans (Glycine max L. Mevr.) were studied using a completely randomized block design under greenhouse condition. Treatments consisted of five levels of trifluralin (0, 1, 2, 3, 4, mg a.i/kg soil), three levels of iron (0, 5, 10 mg a.i/kg soil), and five cultivars of soybeans (Williams, Clarck, Pershing, KW505, SRF 450). Results indicated that increase in trifluralin concentration significantly decreased plant height, stem diameter, leaf area, dry weight of shoot and root and root nodules in all cultivars for all levels of iron. However, root to shoot dry weight ratio was increased. Trifluralin at zero iron level, caused significant reduction in chlorophyll and iron concentration of shoot in Williams, Pershing and KW 505, but did not have any effect on Clarck and SRF 450. Trifluralin application at 5 and 10 mg iron/kg soil did not significantly affect chlorophyll and iron concentration. With trifluralin application, reduction rates of chlorophyll and iron concentration in cultivars were significantly different. The highest sensitivity was observed in KW505 and Pershing and the lowest in Clarck and SRF450. Application of 5 mg iron/kg soil, significantly increased leaf area of KW 505, but did not have any significant effect on trifoliate leaf number. Application of 5 and 10 mg iron /kg soil significantly increased shoot dry matter in KW 505 and Williams respectively, but root to shoot dry weight significantly decreased with application of 5 mg iron/kg soil. Application of 5 mg iron/kg soil significantly increased root dry weight of KW505. Iron application did not have any significant effect on dry matter and number of root nodules. However iron application significantly increased leaf chlorophyll concentration of Williams, Pershing and KW 505 and shoot iron concentration of all cultivars.