There is no data on the effect of maximum and minimum premedication dose of atropine on work performance of heart and amount of Fractional Shortening (FS) in rabbit echocardiography, so this study was designed for evaluation of probability of the above effect.24 White New Zealand rabbits, clinically healthy with no consideration in sex and age, weighting 1.5±0.3 kg in four groups consisting six animals for access to the purpose of study were evaluated.Injection of atropine with maximum and minimum dosage for first and second groups and injection of distilled water for third and fourth groups were observed. Calculations of FS for left ventricles in statutory times were done by echocardiography with no knowledge about injection solution.Determinations for Mean, Standard Deviation, Minimum and Maximum amount for FS were 37.29, 3.355, 30 and 43 respectively. Determinations for Mean and Standard Deviation for FS in group one, before injection and in minutes 2, 5, 15 and 30 after injection were 36.33±3.011, 35.33±2.944, 36.17±4.119, 36.83±3.251 and 37.00±2.449 respectively. Determinations for Mean and Standard Deviation for FS in group two, before injection and in minutes 2, 5, 15 and 30 after injection were 39.17±2.994, 39.00±3.347, 36.50±3.017, 35.50±6.025 and 36.83±5.811 respectively. Determinations for Mean and Standard Deviation for FS in group three, before injection and in minutes 2, 5, 15 and 30 after injection were 36.17±2.483, 33.83±3.061, 34.33±2.805, 35.00±2.828 and 33.67±4.885 respectively. Determinations for Mean and Standard Deviation for FS in group four, before injection and in minutes 2, 5, 15 and 30 after injection were 37.50±4.550, 34.17±4.622, 37.17±3.125, 36.50±1.225 and 37.50±2.811 respectively.As a conclusion, Stability of heart performance was observed after using maximum dose of atropine.