Background: This study aimed to determine the effect of blood groups on dyadic adjustment, quality of life, and health anxiety in nurses. Methods: In this causal-comparative research, 200 married nurses working in the health care centers of Iranshahr City were selected by simple random sampling using Cochran’, s sample size formula in 2019. Data were collected using Spinner’, s marital adjustment questionnaire, War and Sherborn’, s quality of life questionnaire, and Salkovskis’,health anxiety inventory. Results: To investigate the difference among the groups, SPSS was applied, and multivariate and one-way analyses of variance were run. Amongthe subscales of dyadic adjustment, only the mean score of dyadic cohesion was higher in blood group A than in groups B and AB. Among the subscales of life quality, the mean score of affective role play was higher in blood group A than in groups B and AB, the mean score of social function was lower in blood group B than in blood group O, and the mean score of general health was higher in blood group A than in group B. However, the mean score of health anxiety was lower in blood group A than in blood group B. Conclusions: Given the relationship between health anxiety, marital compatibility, and quality of life with blood types among married nurses, the present study results can help increase people’, s awareness about at-risk blood types. As a result, vulnerable groups can be screened and trained to improve their lifestyles, healthy behavior, and environment to live a better life.