Introduction: In recent years, due to the increase in the use of pesticides in agricultural products and their entry into the human food chain, there has been an increase in concerns about food health among people, researchers, and international organizations. Therefore, this research aims to validate the analysis method of 25 pesticide residues in rice using LC-MS/MS device. Methodology: In this research, a simultaneous analysis method was set up to determine the amount of 25 types of commonly used pesticides in rice using liquid chromatography with sequential mass spectrometry (LCMS/ MS). Sample preparation steps were performed based on QuEChERS optimized method, using buffer extraction and purification based on the d-SPE method. LC-MS/MS technique with electrospray ionization (ESI) in positive mode was used for the simultaneous determination of 25 types of pesticides in a 20-minute run and in the MRM program. Results: The limit of quantification (LOQ) and limit of detection (LOD) for all pesticides were 25 and 8 ng/g, respectively. 30 samples of rice imported from Tehran were collected and analyzed. Out of the total number of 30 samples, 9 samples, i. e. 30% of the samples, were contaminated with Triazophos, Phoratesulfoxide, Fosthiazate, Formetanate, Fonofos, and Cyphenothrin toxins,Of these, 4 samples were higher than the MRL determined by the National Governor’, s Organization of Iran. Conclusion: The results of the present study show that pesticides were found in all the samples examined in this research, and considering the permissible limit of these toxins according to the standard and the consumption of rice in the food basket of most people in the region, it is a threat to consumers.