The purpose of this study is to construct and validate a questionnaire of professional competencies of teachers of media thinking and literacy. The research method is survey research and its statistical population also includes 100 teachers who have experience in teaching thinking and media literacy in Birjand. In the present study, the sample size based on Morgan table is 80 people and simple random sampling method has been used for sampling. The data collection tool was a questionnaire and the data were analyzed with the help of SPSS software and using construct validity with exploratory factor analysis by principal components method and orthogonal rotation by Varimax method. 16 factors have been identified in the questionnaire that has a specific value higher than one. Items with a subscription rate of less than 0. 5 were removed from the questionnaire and exploratory analysis was performed twice more on the remaining items, which were eventually reduced to 7 components, which were obtained as components of the final questionnaire. They had both theoretical and logical justifications. The reliability of the questionnaire was also calculated using Cronbach's alpha coefficient (0. 94),which indicates the acceptable consistency of the questionnaire. The results of these analyzes showed that this questionnaire had seven factors 1) Behavioral competencies,2) Media knowledge,3) Teaching skills,4) Thinking skills,5) Media skills,6) Emerging media literacy,and 7) Pedagogical competence It has good reliability and validity.