Introduction: In some jobs, physical activity might be performed in high noise pollution, which can affect the heart rate. The aim of this study was to investigate the effects of noise exposure and physical activity on heart rate in hot and humid conditions.Methods: This experimental study was performed on 14 healthy males in hot and humid climatic chamber (35°C, 75% RH). Heart rate was measured at rest, sitting, low workload, moderate workload and heavy workload in non-noise exposure and noise exposure trials in 65, 75, 85 and 95 dBA levels.Results: In hot and humid weather conditions, there was a significant relationship in heart rate average between conditions of sitting, low, moderate and heavy workload, in nonexposure to noise and noise exposure at level of 65, 75, 85 and 95 dB (A) (P<0.05). In all activity levels, the heart rates significantly increased with increasing the sound intensity. There was more increase in heart rate in higher than the threshold limit noise level in moderate and heavy workloads.Conclusions: According to the additive effects of noise exposure on heart rate in hot and humid conditions, workload levels are considered higher in hot and humid conditions compared with thermal comfort conditions, if there is exposure to noise over 85 dBA.