There are many rainfall variations in dry lands so the application of a Supplemental Irrigation (SI) system may result in increased seed yield. Dry land pulses are grown without chemical fertilizers. Since the root of pulse has a nitrogen-fixation ability, improving the biological conditions and moisture content can increase seed yield. The effect of seed inoculation with phosphate-solubilizing-bacteria (PSB) and Arbascular Mycorrhizae fungi (AM) on yield and water use efficiency of lentil was examined under conditions of SI. This study was performed at the Rainfed Agricultural Research Station in Northern Khorasan over two years with four replications in a split plot design. Three levels of irrigation were applied as the main factor including: no irrigation, a single irrigation at planting and one at the flowering stage. The application of biological materials at three levels as sub factor was considered including: no use of biological materials, seed inoculation with PSB, and a mixture of PSB and AM. The results showed that SI had significant effect on some traits including seed yield, biological yield, harvest index (HI), evapotranspiration (ET), water use efficiency (WUE), and N and P seed content. Biological materials only caused a significant effect on ET, WUE and P seed content and no significant interaction was observed between these factors in the two years. Irrigation at flowering stage increased seed yield 20% more than irrigation at the planting stage. The use of biological materials in conjunction with irrigation had no effect on seed yield. Water use efficiency in rainfed conditions diminished with the use of PSB while using of mycorrhizal fungi was important, especially due to positive effects on water use efficiency and, throughout mycorrhizae, enhanced sustainability of plant production.