Nickel (Ni) has recently added to the list of essential nutrients. Ni is needed for plants in low concentrations but it is toxic at high concentrations. Considering the importance of iron (Fe) and that Fe deficiency is one of the main nutritional problems of the Iranian people's, this study was carried out to investigate the interaction effect of Ni×Fe on concentration and uptake of Ni and Fe by corn plant (Zea mays L). A greenhouse experiment was conducted within the framework of factorial randomized complete block design (RCBD) in a calcareous soil with Ni treatments at four levels (0, 10, 50 and 100 mg Ni kg-1 soil) from NiSO4 and Fe from FeSO4 and Fe-EDDHA at five levels (0, 10 and 20 mg Fe kg-1 soil), according to two source on corn plant (single cross 704). After 90 days, plants harvested and Ni and Fe concentration of shoot and root were measured. According to the results, with increasing applied Ni levels, shoot Ni concentration significantly increased at levels 10, 50 and 100 mg Ni Kg-1 soil, respectively, 18. 78%, 41. 106%, 97. 116% compared to the control. With increasing applied Ni levels, root Ni Concentration increased respectively, 1. 27, 4. 63, 9. 18 compared to the control. With increasing levels of applied Ni in the soil, root Fe concentration significantly (P<0. 05) decreased at levels 10, 50 and 100 mg Ni Kg-1 soil, respectively, 8. 27%, 16. 1% and 32. 7%. With increasing levels of applied Ni in the soil, Fe concentration of root significantly (P<0. 05) decreased at levels 50 and 100 mg Ni Kg-1 soil, respectively, 8. 7% and 9. 3%. With the increase in Fe levels, translocation factor of nickel significantly increased 44. 8 and 48. 9 percent at 10 and 20 mg kg-1 soil when FeSO4 was Fe source and at 10 mg Fe kg-1 from Fe-EDDHA 55. 1 percent respectively. According to the results, interaction of Ni and Fe was affected by Fe fertilizer sources.