Introduction: Diabetes is a metabolic disease that its prevalence is increasing day by day. Diabetes patients are encounter with endothelial dysfunction. The purpose of this study was to investigate the effects of aerobic interval training on flow- mediated dilation endothelial brachial, nitrite/ nitrate (NOx) and secondary outcome in type 2 diabetes patients.Method: In this quasi-experimental study, thirty diabetic patients referred to diabetes and metabolic centers of Tehran recruited in the summer of 1393.All the subjects had type 2 diabetes and well controlled hypertension. They divided into two equal intervention and control groups. Training program underwent 3 session in week for 3 month. The biochemical parameter of glucose, total cholesterol, triglyceride and HDL, HbA1c, insulin and NOx was measured. The effect of training intervention was analyzed with two way- ANOVA by SPSS 18 version.Results: Exercise training caused a significant increase in VO2 peak (P<0.005), NOx (P<0.004), while significantly reduced glucose level (P<0.023). However, ten weeks of interval training had not any significant effects on Flow Mediated Dilation (P<0.189).Conclusion: Arobic interval training is an appropriate intervention for development of physical fitness factors in type 2 diabetes patients. However, this exercise training can’t improve FMD index. It seemed that duration of exercise training was more than the initial adjustment phase is functional, and which entry to structural adaptation.