Iranian South Medical Journal
Year:2018 | Volume:20 | Issue:6 |Papers Count:7

Background: Selenoprotein P (Sepp1) is the major selenoprotein in plasma and contains over 50% of the total plasma. The direct and indirect effects of Sepp1 on anti-oxidant defense can be responsible for its role in cancer risk. One of the common polymorphism of SEPP1gene is the Ala234Thr single nucleotide polymorphism (SNP) in coding region. In this research we studied the association between SEPP1 Ala234Thr (rs3877899) polymorphism with the risk of breast cancer. Materials and Methods: In this case-control study, 162 patients with breast cancer were compared with 227 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes. Determination of DNA genotyping in SEPP1 Ala234Thr (rs3877899) polymorphism was performed by the polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP) by using MwoI enzyme. Statistical analysis was performed by using the MedCalc program and χ 2-test. Results: The genotype frequencies were significantly different between the cases and controls. AA homozygosity subjects were more at risk for breast cancer than others (OR=3. 23; 95%CI, 1. 79-5. 84; p=0. 001). Conclusion: Based on the results of this study, The Ala234Thr polymorphism of the SEPP1 gene may be associated with the potential for breast cancer in the female population of northern Iranian women. However, studiesneed to be conducted in larger populations to confirm these results.

Background: The aim of this study was to investigate the possible role of Protein Kinase Cᵧ (PKCᵧ ) in morphine tolerance and induced hyperalgesia following repeated morphine administration in male rats. Material and Methods: Rats were divided into 4 groups for testing. The groups consisted of control (saline), morphine tolerance and hyperalgesia, metformine, morphine tolerance and hyperalgesia + metformine. First morphine (10mg/kg, s. c) administrated daily up to 14 days subcutaneously. Hargreaves' behavioral test was done before (control) and after the first dose of morphine on first day and then on day14th after last injection to evaluate morphine tolerance. To consider morphine hyperalgesia PWL was assessed by Hargreaves test before and after first morphine injection on first day and then on day 14th before last injection of morphine and on day16th two days after last injection of morphine. The expression of spinal PKCᵧ measured on day 14th and 16th. Metformin 100mg/kg, co-administrated with morphine daily up to 14 days then PWL and PKCᵧ expression were assessed as mentioned above. Results: The results showed significant tolerance and hyperalgesia on day 14th and 16th in compare to control. PKCᵧ expression also increased significantly on day 14th and 16th in compare to control but there was no significant differences in compare of day 14th and 16th. Co-administration of metformin and morphine up to 14 days increased PWL in morphine tolerance and reduced hyperalgesia and PKCᵧ expression. Conclusion: Results showed that increasing of spinal PKCᵧ expression in chronic morphine administration may involve in morphine tolerance and hyperalgesia.

Background: Toxic elements can seriously affect prostate gland. One of the toxic material with a high prevalence is lead. On the other hand, medicinal herbs such as wheat sprout can be used for reducing the heavy metal toxicities. This study investigated the effects of wheat sprout extract on histological and histometrical structure of prostate gland in rats exposed to lead acetate. Materials and Methods: Thirty rats were divided randomly into six groups: G1 (control group) received 1 ml/kg/day of normal saline, G2 received 20 mg/kg/day of lead acetate, G3 and G4 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract respectively, G5 and G6 received 100 mg/kg/day and 200 mg/kg/day of wheat sprout extract respectively with 20 mg/kg/day of lead acetate. After 35 days, rats were sacrificed and blood and prostate tissue samples were collected for histomorphological and histochemical studies. Results: Results showed a significant decrease in epithelial height of secretory units (P= 0. 02) and percentage of parenchyma to stroma (P= 0. 036) in lead group, compared to that in control group. On the contrary, a significant increase was seen in epithelial height in group receiving wheat sprout extract (200 mg/kg/day), compared to control group (P= 0. 033). Serum testosterone and antioxidant activity (AOA) showed a significant decrease in lead group, compared to control group (P< 0. 001). Conclusion: Wheat Sprout extract is effective in improving the tissue structure of the prostate gland in rats exposed to lead acetate.

Background: Due to the prevalence of periodontal disease in diabetic patients and known oral complications of diabetes, also due to the lack of information of diabetics towards periodontal disease and absence of any research in assessment of these two diseases in Bushehr city; this research has been done with the aim of assessment of knowledge, attitude and practice of diabetic patients towards periodontal disease. Materials and Methods: This descriptive-analytic study was carried out by a questionnaire. The assessed population consisted of diabetic patients above 40 years of age (n=150), with diabetic type 1 or 2, controlled or uncontrolled, regardless of taking medication, having at least 10 teeth and were selected randomly. The questionnaire consisted of 25 questions which 5 of them were in relation to knowledge, 8 questions in relation to attitude, 6 questions in relation to practice and others were related to demographic data. Data collected from the questionnaires were coded and entered SPSS 18 software and was analyzed with the use of statistical analytic tests. Results: 51. 30% of diabetic patients had a good level of knowledge and 62% had a good attitude level. Also, 44. 70% of diabetics had a poor level of practice. There was a positive coefficient correlation between knowledge and attitude, knowledge and practice and attitude and practice. Conclusion: This study indicated that more than half of diabetic patients in Bushehr city had a good level of knowledge and attitude but their practice level was poor. Therefore, else methods are needed to improve patients’ practice level.

Background: New technologies change different fields including medicine. One of these technologies is telemedicine. Telemedicine can improve equity of access to health care and enhance the quality of care. Evaluation of physicians’ knowledge and attitude has a key role in acceptance of this technology. The aim of this study was to assess the specialist physicians’ perspectives about telemedicine and barriers in Tabriz teaching hospitals. Materials and Methods: This study is a cross sectional that was done in 2017. This study was done by using a questionnaire that consists of 4 sections (Knowledge, Attitude, Barriers and usage rate). 117 specialist physicians of Tabriz teaching hospitals were selected based on convenience sampling method and participated. Results: Knowledge of %33. 55 of physicians about telemedicine was low. About %68 of physicians believed that using telemedicine has caused an improvement in health service. %45. 3 of physicians used telemedicine that the most used related to consultation by telephone. Lack of culture for usage of telemedicine, lack of knowledge about this technology and lack of facilities were important barriers of establishing telemedicine. Conclusion: This study was showed the Knowledge and usage rate of Specialist Physicians about telemedicine was low and acceptance rate of telemedicine was high. For eliminating of barriers and appropriate implementing of this technology, specialist physicians require comprehensive education for orientation with telemedicine services and also essential infrastructures including culturaland technical ones must be fully prepared.

Background: Gestational diabetes can leads to macrosomy, fetal abnormalities, increase prevalence of hypertension, increasing unreasonable morality. Diet plays a very important role in the consequences of pregnancy in these patients. The purpose of this study was to assess the effect of diet based on body mass index (BMI) on pregnancy outcomes in diabetic pregnant 20-35 year old women referring to Arash hospital. Materials and Methods: In this randomized double-blind clinical trial, 70 diabetic pregnant women 20-35 year old were divided in two groups as case and control. Control group was on diet with 30 kilo calorie per kilogram energy without consideration BMI. In this way, The case group, with BMI 19-24. 9 got 30 kilo calorie per kilogram energy and with BMI 25-29. 9 got 25 kilo calorie per kilogram energy. SPSS version 23 software was used for analyzing and (P<0. 05) was considered significant. Results: There was no significant difference between case and control group about mean of increasing weight end of pregnancy (p=0. 3), The birth weight (p=0. 05), Insulin requirement (p=0. 7), macrosomia (p=0. 2), hypertension (p=0. 1), intra uterine growth retardation (p=0. 3), gestational age in delivery (p=0. 3), infant blood calcium (p=0. 3) but infant blood sugar in case group was less than control group significantly (p=0. 01). Conclusion: Calorie restriction based on BMI in case group compared to control group was not effective about more pregnancy outcomes such as average weight gain at the end of pregnancy, Insulin requirement, weight of newborns at birth, macrosomia baby birth, Having high blood pressure, intra uterine growth retardation, gestational age in delivery, infant blood calcium. but infant blood sugar in case group was less than control group significantly.

Background: Arsenic occurs naturally in the earth’ s crust. Although arsenic is useful for various industrial, agricultural, medicinal and other purposes, it exerts a toxic effect in a variety of organisms, including humans. Arsenic could be entered into the environment through anthropogenic (produced directly by human activities) and natural (weathering and leaching from rocks and mineral layers of the earth and sedimentation) sources. This study evaluates the source of arsenic contaminations in the south of Kerman state by preparing the arsenic distribution map. Materials and Methods: In south of Kerman Province, with a surface of ca. 5000 km2study, results of assaying the 1804 stream sediment samples for arsenic contamination are used for spatially modeling. Empirical Bayesian kriging (EBK) modelling has been applied to assess the prediction of arsenic contamination for other non-sampling. Results: The level of arsenic concentration is more than the standard value (12 ppm) in north-western part of the area. This contamination can cause several diseases such as skin, Bladder and Lung cancers, diabetes and Cardiovascular in these areas. Therefore it’ s necessary to evaluate the frequency of these diseases in the contaminated area. Conclusion: Comparison of contaminated area with the location of mineral deposits, mines, chemical industries and agricultural area shows that the source of this contamination could not be anthropogenic and natural source is more probable. Because the spatial modeling is prepared based on the stream sediment samples, it is necessary in the next step, to sample from soil, surface and underground water of contaminated area.