IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)
Year:2016 | Volume:19 | Issue:5|Papers Count:16

Background: Nowadays, composite scaffolds with some desired characteristics have a numerous applications in hard tissue engineering. In present study, the role of composite hydroxyapatite - gelatin was examined in both alone and coated by Bone Marrow Stromal Stem Cells (BMSCs) conditions in the process of healing bone defects, reduction of time repair and the immune response of body by laboratory studies (in vitro) and in vivo on the skull of adult rats as well.Materials and Methods: In present study, nano-hydroxyapatite powder and gelatin were used to provide nano-hydroxyapatite-gelatin scaffold, BMSCs were isolated by Flushing method. Fifteen adult male Wistar rats weighing 250-200 g were used. Studing groups included bone defect with hydroxyapatite-gelatin scaffold, bone defect with hydroxyapatite-gelatin with BMSCs and bone defects without scaffolding as a controlwhich were examined after a week and a month after surgery. MTT assay was used in order to evaluation of biocompatibility of scaffolds. To confirm the healing progress trend and the presence of inflammatory cells we used hematoxylin-eosin and we used Masson's trichrome staining in order to study of synthesis of collagen fibers.Results: The results of MTT showed that the scaffold has no toxic effects on stromal cells. The first signs of ossification in hydroxyapatite-gelatin with BMSCs cells group, appeared in the first week. However, in the fourth week, ossification was completed and the scaffold remaining was found as embedded islands in the spongy bone tissue. The greatest number of lymphocytes was observed in the experimental group after one week of planting scaffold.Conclusion: it seems that Hydroxyapatite-gelatin scaffold coated with BMSCs cells has a potential role in the healing process of bone and it can be suitable as a therapeutic strategy to repair extensive bone lesions.

Background: Benzo [a] pyrene (BaP) is a high molecular weight polycyclic aromatic hydrocarbon (PAH) that have high carcinogenic effects. So, the aim of this study was to assess the effects of benzo [a] pyrene (BaP) on liver EROD enzyme activity induction and liver DNA breakage in Java Medaka.Materials and Methods: twenty three Java Medaka were transferred to 10 L glass aquarium and exposed to benzo [a] pyrene concentrations of 0.5 mg/L (Low concentration), 1.5 mg/L (median concentration), 5 mg/L and 0 mg/L (DMSO solvent control), with semi-static renewal technique during 7 days. Fish liver biopsy was performed in seventh day and a part of the samples was immediately homogenized and were used to measure EROD enzyme activity. Another part of the liver samples were transferred to -20oC to use for DNA integrity assay. Data were analyzed by using ANOVA and Duncan tests. The p value £ 0.05 was considered as a level of statistical significance.Results: A significant increase in EROD anzyme activity was observed between the experimental treatments compared to the control group. Although the fishes that exposed to the highest concentration (5 micrograms per liter) of Benzo [a] pyrene had higher DNA breakage. Nevertheless, there was no significant difference between the treatment groups compared to the control group.Conclusion: benzo [a] pyrene combination increased hepatic EROD activity, the enzyme in the initial phase of PAH detoxification, in Java Medaka. However, it seems that Java Medaka fish have a DNA repair and preventive mechanisms from liver DNA breakage.

Background: Transplantation of Schwann cells can facilitate the regeneration of peripheral nerves. The aim of this study was to comparison of Schwann cells transplantation effect with autograft model in peripheral nerve regeneration in animal model.Materials andMethods: 20 maleWistar rats were randomly were divided into 3 groups: control, Schwann cells transplantation and autograft model. In the control group, a 10 mm segment of the left sciatic nerve was removed and a silicone tube replaced into this nerve gap. In the Schwann cells transplantation group, after placing the silicone tube were transplanted into the tubeabout 500, 000 Schwann cells. In the autograft model group, 10 mm segment of the left sciatic nerve is removed and it was implanted to the two nerve endings after reversing.12 weeks after surgery we evaluated the number of axons, the number of blood vessels and the restored myelin sheath thickness.Results: Histological analysis by using one way ANOVA showed that the number of axons and the thickness of myelin sheath in autograft model group was significantly greater than the other groups, and in the Schwann cells transplantation group was significantly greater than the control group. Moreover, the number of restored blood vessels in the Schwann cells transplantation group was significantly greater than the other groups (P<0.05).Conclusion: The results show that Schwann cells transplantation is effective in peripheral nerve regeneration and it may be a good alternative to autograft method.

Background: hyper-methylation in CpG Island is one of the major mechanisms in gene silencing. In many cancers, different genes are experiencing CIHM (CpG island hyper methylation). P14 / ARF regulatory factor, involved in negative regulation of the cell cycle through the effect on P53 factor pathway. On the other hand, Interleukin 17 can be increased methylation by inflammatory effects. The purpose of this study was to study the P14/ARF gene promoter methylation and effect of Interleukin-17 on this methylation among breast cancer patients in the south of the country and its comparison with healthy people.Materials and Methods: in this case–control study, peripheral blood of 40 patients with breast cancer who were referred to hospitals in Shiraz and 40 healthy women was used to DNA extraction by using salt out and K proteinase. Control subjects with a family history of cancer or autoimmune diseases were excluded from the study. We used PCR-RFLP method In order to study of Interleukin-17 gene polymorphism, and MSPCR method was used to study of P14/ARF gene promoter methylation. The results of the study were studied by using SPSS software, Arlequin, chi-square and Hardy-weinberg equilibrium test was used respectively.Results: findings confirms that there was a significant association between P14/ARF gene promoter methylation and disease and mentioned gene promoter was less methylated in healthy subjects compared to patients (p<0.05). On the other hand, there is a significant association between GG genotype in IL17 F gene polymorphisms and P14 /ARF gene methylation (P<0.05).Conclusion: P14/ARF gene promoter’s methylation play an important role in breast cancer. Due to the decline of P14P14/ARF gene promoters methylation and its association with this disease, seems that it could be used as a biomarker for diagnosis.

Background: Remote Ischemic Preconditioning (RIPC) improves exercise performance, and since this phenomenon has two phases, the aim of the current study was to investigate the delayed effects of remote ischemic preconditioning on cardiopulmonary function in athletes and non-athletes.Materials and Methods: 25 male and female students were studied in two main athletes and non-athletes groups. RIPC was induced by using 3 cycles of alternative 5 minutes ischemia and 5 minutes reperfusion at arms of participants. Cardiopulmonary tests were measured before, after and 24 hours after inducing remote ischemic preconditioning. Maximum oxygen consumption (VO2max) estimated by using queen steps test.Results: Analysis of data demonstrated that delayed RIPC in non-athletes group caused significant improvement in Forced Expiratory Volume in one second (FEV1) and Maximum Voluntary Ventilation (MVV) and noticeable improvement in some other parameters of pulmonary function tests. Moreover, it decreased systolic blood pressure and heart rate and decreased lactate release in both groups especially athletes group but it had no significant effect on VO2max of both groups.Conclusion: Delayed RIPC improves cardiovascular function of athletes and pulmonary function of non-athletes subjects. Thus, it can be considered as a good replacement for doping to improve sports performance of subjects in sports tournaments.

Background: The study of the molecular dynamics of proteins based on internal motions is crucial for understanding the mechanism of protein function. Internal movements of proteins play an important role in both protein folding processes and the mechanism of action of enzyme. Knowledge about these movements will help us in understanding these processes correctly. Osmolites are small organic molecules which are utilized by the cells of all organisms, except hallobacteria. Osmolites are produced under extreme stressful conditions in order to help in stabilizing macro-molecules and retaining their biological function. They interact directly with macromolecules, but they exert their effect only when the characteristics or conditions of the solvent present around the cell change. Therefore, their presence affects the stability of the protein indirectly.Materials and Methods: In this work we studied the effect (s) of the osmolite Sorbitol on the dynamics of Hen Egg White Lysozyme, and through our study we used NMR Hydrogen-Deuterium Exchange to characterize the effect (s). We performed spectrometery in two different conditions, in the absence and presence of sorbitol. The extent of peptide hydrogen exchange was assigned as a function time and in a decreasing manner in the intensity of the peak corresponding to amino acids. The resulting TOCSY spectra were assigned using SPARKY software, as well as we integrated the peaks using this software.Results: The speed of hydrogen exchange, hydrogen atoms of the peptide bond, will give us information about the local structural oscillations where the exchange is taking place. The rate of hydrogen exchange varies from one amino acid to another, and the intensity of the peak of these hydrogen decreases as time passes by.Conclusion: We found that the presence of sorbitol causes a decrease in proton exchange rate, and since there is no noticeable chemical shifts in the peaks of the spectra, in the presence or absence of sorbitol, we can conclude that sorbitol did not cause any change in the three dimensional structure. It seems that the decrease in the rate of exchange is a representative of a decrease in the accessible surface area or to strengthening of hydrogen bonds, and sorbitol caused one of these two possibilities.

Background: Accumulation of heavy metals and increasing their concentration and reaching them to the danger range can threaten human health by entering to food chain. Thus, continuous monitoring of pollutants consentration in agricultural products is important. So, the aim of this study was to investigate the heavy metals concentration and risk assessment resulting from them in agriculture product with two types of irrigation in farms of Dayyer city which is one of the counties in Boushehr province.Material and Methods: Samples of agriculture product were harvested in three in three categories vegetables included: leafy (lettuce, spinach, cabbage), tuber (onion, potato), and fruit (tomato, green pepper) from farms of Dayyer city with two types of irrigation included River Water Irrigation (RWI) and Ground Water Irrigation (GWI). Totally 320 samples were analyzed by using (ICP-OES -Spectrum Arcos) after acidic digestion steps and extraction of heavy metals from samples. Then, the obtained results were analyzed by diffrent equations and statistic softwares.Results: results showed that the concentrations of metals were 68.75 (47.71), 277.62 (196.12), 32.68 (18.31), 42.19 (17.09), 1.76 (0.87) and 12.04 (5.83) mg kg-1 for Zn, Mn, Cu, Cr, Cd and Pb in RWI (and GWI), respectively in irrigated farms with river water (farms irrigated by ground water). The concentration of heavy metals was significantly higher in the soil of farms which irrigated with river water than those which irrigated with ground water (P<0.05). Also, results showed that in farms which irrigated by ground water, the highest Target hazard quotient (THQ) have been reported in spinach with mentioned values; Zn (0.1150), Cd (1.2846), Cu (0.2030) and Pb (1.9141).Conclusion: The findings of this study showed that the concentration of heavy metals in soil and agricultural products which irrigated with contaminated water with urban and industrial sewage was higher than soil and agricultural productswhich irrigated with ground water. Also results of this study showed that the hazard for consumers health in agricultural products that were irrigated with water contaminated by industrial and urban sewage was higher than which irrigated with ground water. The researchers of this study suggest that we seriously should be avoided from entering the urban and industrial sewage to water which irrigate the agricultural products.

Background: The aim of the present study was to examine the association between mental health and PTSD with mediating role of personality traits in earthquake victims of Bushehr.Materials and Methods: The population was all of persons who had experienced the 6.20 earthquake in Shonbeh city of Bushehr province in 2011. Among them 400 persons were selected by using multistage random sampling method. The research instruments were Personality Traits Inventory (NEO), Mental Health Questionnaire (GHQ), and PTSD Scale. Evaluation of proposed model was performed by using the structural equation modeling.Results: The fit indices indicated that the proposed model was fit with data. Results revealed that there was an association between mental health with personality traits directlyand indirectly through personality traits with PTSD. Among personality traits only neurotic and openness were significant.Conclusion: in fact, the findings of this study showed that having mental health with personality traits is a solution to relieve PTSD symptoms, and in people with mental health with positive personality traits such as openness to experience decrease the risk of disorder and severity of PTSD symptoms among them.

Background: Nowadays, volatile organic compounds (TVOCs) are considered as one of the most well-known air pollutants, especially in the indoor environments. The aim of this study was to measure the amount of TVOCs in indoor and fresh air around the intended kindergartens and investigation the association between indoor and outdoor concentrations.Materials and Methods: The measurement was done in four kindergartens in Ahvaz city in five days between 9 to 10 o' clock in the morning. Overall, 40 samples were collected. The PhoCheck PID Detector portal device was used for activity sampling in this study. Also, the results were reported by SPSS16 Software and descriptive statistics.Results: The mean of measured TVOC in the indoor and outdoor air were 50mg/m3 (SD= ±74) and 495 mg/m3 (SD= ±310) respectively. The analyses conducted by the paired-samples t-test and it indicated that the amount of fresh air is not effective on the indoor environment values.Conclusion: The measured values in indoor air of these kindergartens depend on internal factors and appropriate ventilation is slightly had been effective.

Background: Work is an important source for establishing livelihoods and social occasions; however, it can also damage on person's health. The aim of the present study was to study of association between health locus of control and health promoting behaviors among employees of Bushehr University of Medical Sciences.Materials and Methods: This cross-sectional study was conducted on 208 employees of Bushehr University of Medical Sciences by using convenience sampling method. Data were collected by using standard questionnaires of health-promoting lifestyle and multidimensional health locus of control. Data analysis done by SPSS software version 20. Descriptive indicators and linear regression test was used.Results: Among regression models which related to health promoting behavior and its six dimensions, only there was a significant association between total behavior, physical activity and interpersonal relationship dimensions. Between demographic factors and health locus of control only internal health locus of control explained the health promoting behavior and it explained health promoting behavior changes within 3.2% (R2=3.2%). None of the three health locus of control explained physical activity dimensions, while internal health locus of control explained interpersonal relation dimension positively and chance locus of control explained interpersonal relation dimension negatively and explained interpersonal relation changes within 4.9% (R2=4.9%).Conclusion: Due to the effect of internal health locus of control on health promotion behavior and interpersonal relationships, we should try to internalize locus of control by performing counseling programs to step toward improving employee health by improving behaviors related to health.

Background: Work is an important source for establishing livelihoods and social occasions; however, it can also damage on person's health. The aim of the present study was to study of association between health locus of control and health promoting behaviors among employees of Bushehr University of Medical Sciences.Materials and Methods: This cross-sectional study was conducted on 208 employees of Bushehr University of Medical Sciences by using convenience sampling method. Data were collected by using standard questionnaires of health-promoting lifestyle and multidimensional health locus of control. Data analysis done by SPSS software version 20. Descriptive indicators and linear regression test was used.Results: Among regression models which related to health promoting behavior and its six dimensions, only there was a significant association between total behavior, physical activity and interpersonal relationship dimensions. Between demographic factors and health locus of control only internal health locus of control explained the health promoting behavior and it explained health promoting behavior changes within 3.2% (R2=3.2%). None of the three health locus of control explained physical activity dimensions, while internal health locus of control explained interpersonal relation dimension positively and chance locus of control explained interpersonal relation dimension negatively and explained interpersonal relation changes within 4.9% (R2=4.9%).Conclusion: Due to the effect of internal health locus of control on health promotion behavior and interpersonal relationships, we should try to internalize locus of control by performing counseling programs to step toward improving employee health by improving behaviors related to health.

An 88-year-old man reffered to Emergency section of Persian Gulf Martyrs hospital, in Bushehr with clinical impression of upper GI bleeding & severe anemia. In the endoscopy section he became agitated during removal of his NG tube and nurse reported that he felt resistance during extraction of his NG tube. After the removal of a NG tube revealed a distal tight simple knot is observed at the distal end rigid tube. After evaluation by upper endoscopy & barium meal disclosed that he is a case of acute gastric volvulus which is a rare & potentially life threatening disease. We believe that anatomic changes due to gastric volvulus are the cause of his knotted NG tube.

With performing the first kidney transplantations in 1950s and 1960s, medical science hopes were raised to find out proper ways for treatment of End Stage Renal Disease or dialysis patients. But regarding to immunologic bases of transplantation and the use of immunosuppressant medicines and their side effects, patients may encounter to severe and inevitable side effects that sometimes may even lead to death. Therefore, in recent years, medical sciences in convergence with technology, pursue a new kind of approach so called "regenerative medicine"; however this method has its own challenges and complexities. But regarding to potential regenerative abilities of aquatic animals such as starfish, it may be possible to overcome on some of these challenges. The results of recent studies on evolutionary processes of human kidney and development and regeneration in starfish and, and presence of path and common cytokines among these processes proves this claim. This article presents some evidences that imply on practical usage of starfish in human kidney regeneration.

Since marine invertebrate have many applications in medicine and biological sciences and pharmaceuticals because of valuable metabolites, in recent years related studies to marine invertebrate’s stem cells culture have dramatically increased. Stem cells Stem cells are considered as the progenitor cells of the body. In fact, culturing of these cells is a developed cell culture that maintains ability to proliferate throughout adult life from birth to death and also these stem cells are able to differentiate into different cell types. Unfortunately related researches to stem cells in marine invertebrates didn’t develop in the same manner of mammals and despite many efforts; mana cell lines have been not derived from these organisms yet. This would be due to the paucity of available resources as well as lack of maintaining of culture conditions that this is mainly due to lack of sufficient knowledge about the mechanisms and systems involved in maintaining their stemness. Many evidences have been reported about the presence of marine invertebrates’ stem cells in hydrozoans, crustaceans, echinoderms and real chordate so far. Understanding the suitable culture conditions and understanding the needs and providing appropriate cellular microenvironment will be the prospect of using this valuable resource. This systematic review will be present an overview of the concepts and activities related to marine invertebrates stem cells.