Background: Health promoting lifestyle (HPL) consists of six aspects including physical activity, nutrition, health responsibility, spiritual growth, interpersonal relations and stress management. This lifestyle promotes health and welfare, induces satisfaction, self-persuasion and self-improvement. Therefore, this study aimed to determine the relation between health-promoting lifestyle behaviors and quality of life among undergraduate students at School of Health, Isfahan University of Medical Sciences.Methods: This cross-sectional study was conducted on undergraduate students at School of Health, Isfahan University of Medical Sciences. A census sample of 81 subjects completed a survey questionnaire.Health Promoting Lifestyle Profile 2 was used in order to measure health promoting lifestyle behavior.Quality of life was measured the Farsi version of QLQ-C30 questionnaire whose validity and reliability have been confirmed before. Data analysis was performed using descriptive and inferential statistical tests in SPSS.Findings: Mean age of participants was 21.12 years. In addition, 54.3% of the subjects were female and 45.7% were male. Spiritual growth and physical activity were respectively the most and least common subscales of HPL practiced. General quality of life of the majority of cases was good (40.7%). The highest and lowest frequencies of health-related quality of life belonged to very good (58.6%) and excellent (9%) scores, respectively. Except for stress management (P=0.05), there were no significant relations between HPL aspects and gender. Female students performed self-care behaviors and relaxation techniques more than male students. Although spiritual growth was significantly related with general quality of life (P=0.006), no significant differences were found between other subscales of HPL and general quality of life. In addition, health-related quality of life was significantly related only with stress management (P=0.03).Conclusion: According to our findings, the majority of students had good general quality of life since 58.6% scored very good and 9% scored excellent in health-related quality of life. Finally, our findings showed that HPL, spiritual growth and stress management were positively related with global quality of life.