Organizational studies show that organizational culture has leverage role in making dramatic developments in competitive, uncertain and changing environments. So Reviewing and analyzing of culture in all organizations, especially in the wide and long one like Payame Noor Universitry (PNU) with millions of people has growing importance. In this regard, the aim of this study is understanding the general status of organizational culture and determining taken attitude of managers considering five dimensions of this important organizational phenomenon. The population in this study was all of university managers in different organizational levels that were 1732 persons. Using simple random sampling, 119 managers were selected for study. Organizational Culture Assessment Questionnaire (OCAQ), Sashkin famous tool, was used to collect data and One-sample t-test was used to test research hypotheses. Ultimately except first hypothesis that showed weakness in change effective management, other next four hypotheses were approved. This result showed that University managers in general, have a positive attitude toward organizational culture, and believe other four dimensions, “goals and perfectionism", “coordinated teamwork", "Cultural Competency in coordination" and "customer oriented" are desirable and effective.