The present study aims at assessing the effect of canopy combination of beech trees and associated species (beech-hornbeam-maple, beech-hornbeam, beech-maple and pure beech) on the basis of litter and soil characteristics in Mazandaran Province, northern Iran. Litter and soil sampling were taken at each beech stand composition using litter bags and plastic bags (30 × 30 × 30 cm), respectively with five replicates under the canopy of central beech trees. In order to study the effect of different seasons on the presence and activity of fauna, sampling was done in spring, autumn and winter. Characteristics of litter quality (carbon, nitrogen, carbon to nitrogen ratio), physicochemical and biological properties of soil (water content, pH, organic carbon, total nitrogen, carbon to nitrogen ratio, earthworm density and biomass, nematodes and microbial respiration) was measured. The results showed that the total nitrogen of soil (0. 48%) and pH (7. 43), biomass (56. 88 mg m-2) and density (57. 05 nm-2) of earthworm, total nematodes (55. 02 in 100 gr soil) in beech-hornbeam-maple stand was higher than other studied stands. In all of the canopy combinations, density (45012) and biomass (46. 08) of earthworms and nematodes (56. 78) had the maximum activities in autumn while the highest microbial respiration of soil (58. 5) was recorded in summer season. Based on the results, beech-hornbeam-maple stands have a great influence on the soil organic and mineral characteristics. The applied results of this research can be used to select the broad-leaved species for restoring the degraded areas in the north of the country.