Backgrounds: Due to their antibacterial effects, herbal remedies have an important role in the treatment of infections; particularly in infected wounds. Increased bacterial resistance to antibiotics combined with increased side effects related to their administration has led many researchers to investigate alternative herbal treatments in recent years. In line with this trend, the objective of this study was to assess the antimicrobial effects of Dr Kamkar's cream on different microbial strains in vitro. Methods: The susceptibility of isolates to the cream was evaluated using well diffusion, the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) methods. 11 microorganisms were analyzed which included 5 strains routinely used to assess antibacterial effects and 6 clinical isolates were obtained from patients' wounds. Based on CLSI (2018) standards, the Kirby& Bauer method was utilized to investigate the sensitivity of the strains to commonly used antibiotics. Results: The herbal cream was effective against 10 strains, while only 1 strain demonstrated resistance. Bacillus subtilis was the most susceptible bacteria to the cream with a zone of Inhibition of 31mm, followed by Bacillus anthracis (25mm), Staphylococcus aureus (22mm), Acinetobacter baumannii (22mm), Staphylococcus epidermidis (20mm), Bacillus cereus (16mm), Pseudomonas aeruginosa and Methicillinresistant Staphylococcus aureus (14mm), Streptococcus pyogenes and Candida albicans (20mm) and resistant to Escherichia coli. MIC findings indicated that the herbal cream had maximal effect against Bacillus cereus, and minimal efficiency against Streptococcus pyogenes and Candida albicans, while MBC results showed maximal effect against Bacillus cereus and minimal effect against Pseudomonas aeruginosa. Results of antibiograms revealed resistance to several antibiotics. Conclusion: According to the results, Dr Kamkar ’ s herbal cream has suitable antimicrobial activity against certain pathogenic microorganisms and can be used alone or in combination with Antibiotics in the treatment of infected wounds.