Effect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells

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Khamehchian Sedigheh; HOSSEINKHANI SAMAN; NIKKHAH MARYAM

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Journal: ALBORZ UNIVERSITY MEDICAL JOURNAL Year:2020 | Volume:9 | Issue:4 Page(s): 367-379

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Title

Effect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells

Author(s)

Khamehchian Sedigheh | HOSSEINKHANI SAMAN | NIKKHAH MARYAM | Issue Writer Certificate

Keywords

MaurocalcinQ1

DoxorubicinQ1

Gold nanoparticleQ1

Cellular uptakeQ1

CytotoxicityQ2

Abstract

Background: Cell penetrating peptides (CPPs) can enter a cell through the cell membrane, and used in the fields of drug delivery, gene therapy, and cancer therapy by their property transporting various molecules into cytoplasm. Gold nanospheres (GNSs) are a useful tool for molecular imaging, because they are not cytotoxic and have high solubility, excellent light scattering property and ease of synthesis. We constructed a drug delivery system by developing gold nanospheres conjugated to MCaUF1-9-C, a CPP derived from Maurocalcine (MCa) scorpion toxin. We examined the applicability of this cell-selective anti-cancer drug delivery system by evaluating its cell-penetrating and cell death activities. Methods: Cell viability of HeLa and MDA-MB-231 cells, against 500 nanomolar concentration of Doxorubicin (DOX)-conjugated Gold nanoparticles (DOX-GNPs, and DOX-MCaUF1– 9-C-GNP) were evaluated using MTT assay. Cell penetrability of MCaUF1-9C peptide conjugated to Gold nanoparticles investigated using of dark field imaging and atomic absorption spectroscopy (AAS). Results: HeLa cell viability was about 87% when treated with DOX-GNP and DOXMCaUF1-9-C-GNP, whereas DOX-MCaUF1– 9-C-GNP induced high Cytotoxicity in MDAMB-231 cells (30%). Dark ﬁ eld imaging demonstrated higher afﬁ nity of MCaUF1– 9-CGNP for the MDA-MB-231 cell compared with HeLa cells. Moreover, atomic absorption spectrometry data analysis showed that 33% of the total amounts of the applied MCaUF1– 9C-GNP were internalized in MDA-MB-231 cells. Conclusion: These results demonstrated that peptide conjugated GNP would be a useful tool for the development of a cell-selective drug delivery system.

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APA: Copy

Khamehchian, Sedigheh, HOSSEINKHANI, SAMAN, & NIKKHAH, MARYAM. (2020). Effect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells. ALBORZ UNIVERSITY MEDICAL JOURNAL, 9(4 ), 367-379. SID. https://sid.ir/paper/414671/en

Vancouver: Copy

Khamehchian Sedigheh, HOSSEINKHANI SAMAN, NIKKHAH MARYAM. Effect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells. ALBORZ UNIVERSITY MEDICAL JOURNAL[Internet]. 2020;9(4 ):367-379. Available from: https://sid.ir/paper/414671/en

IEEE: Copy

Sedigheh Khamehchian, SAMAN HOSSEINKHANI, and MARYAM NIKKHAH, “Effect of Peptide Derived from Scorpion Toxin on Enhanced Permeability of Doxorubicin Conjugated Gold Nanoparticles in HeLa and MDA-MB-231 Cells,” ALBORZ UNIVERSITY MEDICAL JOURNAL, vol. 9, no. 4 , pp. 367–379, 2020, [Online]. Available: https://sid.ir/paper/414671/en

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