Designing of A Multi-epitope Recombinant Protein, Consisting of Several Conserved Epitopes from Hemagglutinin Protein of the H1N1 and H5N1 Strains of Influenza Virus by Immunoinformatics Approaches

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Moghaddam Farnaz; TAGHIZADEH MORTEZA; MANSOURI REZA; TEBIANIAN MAJID; Dabaghian Mehran

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Alborz University Medical Journal Year:2020 | Volume:9 | Issue:4 Page(s): 395-404

Download Full-Text

Persian Verion

View:

555

Download:

Cites:

Information Journal Paper

Title

Designing of A Multi-epitope Recombinant Protein, Consisting of Several Conserved Epitopes from Hemagglutinin Protein of the H1N1 and H5N1 Strains of Influenza Virus by Immunoinformatics Approaches

Author(s)

Keywords

Influenza VirusQ1

ImmunoinformaticsQ1

HemagglutininQ1

Multi-epitopeQ1

Abstract

Introduction: According to marked advances in bioinformatics studies, development of influenza vaccines has been greatly modified in many studies. In this study, we have designed a Multi-epitope recombinant protein, consisting of several conserved epitopes from Hemagglutinin protein of the H1N1 and H5N1 strains of Influenza Virus by Immunoinformatics approaches. Materials and Methods: The registered sequences of Hemagglutinin proteins from H1N1 and H5N1 strains have been analyzed and selected epitopes have been chosen and directed for further analysis. After investigation of immunological and physicochemical parameters, the selected regions were fused together by linkers and sequence of target constructs was determined following codon optimization. The target construct has been integrated into BamHI/XhoI cleavage sites of PET32a+ expression vector. Results: A physicochemical analysis revealed that the designed recombinant protein has 214 amino acid with molecular weight of 22. 451 kDa. The isoelectric point was determined as 7. 25, solubility of 100% and Extinction coefficient of 60170 M-1-cm 1. finally, after codon optimization, the Codon Adaptation Index (CAI) was calculated as 0. 95. Discussion: According to these results, it has been predicted that designed multiepitop recombinant protein has reasonable and unique immunological properties. The expressed protein could be used for future immunological studies against type A Influenza Virus.

Cites

No record.

References

Cite

APA: Copy

Moghaddam, Farnaz, TAGHIZADEH, MORTEZA, MANSOURI, REZA, TEBIANIAN, MAJID, & Dabaghian, Mehran. (2020). Designing of A Multi-epitope Recombinant Protein, Consisting of Several Conserved Epitopes from Hemagglutinin Protein of the H1N1 and H5N1 Strains of Influenza Virus by Immunoinformatics Approaches. ALBORZ UNIVERSITY MEDICAL JOURNAL, 9(4 ), 395-404. SID. https://sid.ir/paper/416012/en

Vancouver: Copy

Moghaddam Farnaz, TAGHIZADEH MORTEZA, MANSOURI REZA, TEBIANIAN MAJID, Dabaghian Mehran. Designing of A Multi-epitope Recombinant Protein, Consisting of Several Conserved Epitopes from Hemagglutinin Protein of the H1N1 and H5N1 Strains of Influenza Virus by Immunoinformatics Approaches. ALBORZ UNIVERSITY MEDICAL JOURNAL[Internet]. 2020;9(4 ):395-404. Available from: https://sid.ir/paper/416012/en

IEEE: Copy

Farnaz Moghaddam, MORTEZA TAGHIZADEH, REZA MANSOURI, MAJID TEBIANIAN, and Mehran Dabaghian, “Designing of A Multi-epitope Recombinant Protein, Consisting of Several Conserved Epitopes from Hemagglutinin Protein of the H1N1 and H5N1 Strains of Influenza Virus by Immunoinformatics Approaches,” ALBORZ UNIVERSITY MEDICAL JOURNAL, vol. 9, no. 4 , pp. 395–404, 2020, [Online]. Available: https://sid.ir/paper/416012/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops