Background and aim: Despite improvements in the properties of composites, glass ionomers and other tooth colored materials, a number of problems still exist in their clinical application including: post-operative sensitivity, microleakage, technique sensitivity and color change.Different methods have been already presented to recuce microleakage. The aim of this study was to compare the microleakage of different restoration methods in posterior CIV cavities.Materials and Methods: On the buccal surfaces of 90 extracted human teeth, due to orthodontic treatment, periodotal problem or impaction, CIV cavities with gingival margin on CEj were prepared.The samples were randomly divided into 5 groups and restored as follows:A: Copalait varnish (Harvard) and amalgam (Cinalux)B: Dentin bonding agent (Exite) and amalgam (Cinalux)C: Dentin bonding agent (Exite) and light cure composite resin (Tetric ceram)D: Resin modified glass ionomer (Fuji II LC) and dentin bonding agent (Exite) and light cure composite resin (Tetric ceram)E: Dentin bonding agent (Exite) and flowable composite (Tetric flow) and light cure composite resin (Tetric ceram)Restorations were polished, thermocycled in 1600 cycles and immersed in 0.5% basic fushin. Samples were then sectioned and studied under a stereomicroscope to evaluate bye penetration. Data were subjected to Kruskal- Wallis test.Results: All groups showed some degrees of microleakage. According to Kruskal-Wallis statistical analysis, differences between groups were significant (P=0.000000< 0.05).Group C showed the maximum and group D Showed the minimum microleakage. Mann-Whitney analysis was used to compare pairs of groups. Results significant differences between groups as (A <B), (A<C), (B>D), (C>D), (D<E), however; no statistical difference was found between (A,D), (A,E), (B,C), (B,E), (C,E) groups.Conclusion: Proper characteristics of glass-ionomer such as its tendency for dentin bonding may be a factor to reduce micro leakage in cervical restorations. It is suggested to use glass-ionsmer under composite restorations in cases that esthetics and secondary caries prevention are important. In cases tath esthetics is not important and economic factor plays a role and the patient is not susceptible to recurrent caries, amalgam is ercommended.