Application of sealer is essential in every obturating technique. Since apical extrusion of sealer during obturation is not uncommon, biocompatibility is of prime importance.
Roth's 800, Grossman's formula with ZOE base, Apexit with calcium hydroxide and Roth's 801 (Apadana Co.) were examined for biocompatibility.
80 male Sprague-Dawley rats with average weight of 800-400 grams were used. All the sealers were mixid according to the manufacture's instructions and placed into polyethylene tubes. Polyethylene tubes were selected as controls. The animals were classified in four groups, each containing 20 rats.
After incisions, the materials were implanted subcutaneously in dorsal surfaces of animals.
The materials were evaluated 4, 10, 30 and 60 days after surgery. After these periods, four rats of each group were selected and sacrificed with high doses of chloroform. After dissecting, the samples were inserted into 10% formalin solution and examined single blindly by oral pathologist in two stages at the Tehran dental faculty. The samples were classified on the basis of inflammation into four groups.
Group 0 (Zero) (without inflammation), group 1 (with mild inflammation), group 2 (with moderate inflammation), group 3 (with severe inflammation). All the results were statistically evaluated by
Kruskal-Wallis and Mann-Whitney tests. In the 4th day, in the control group moderate inflammation were observed which is statistically significant.
In the 10th day, in the control group mild inflammation and in the other three remaining groups sever inflammation were observed which is statistically significant. In the 20th day, in the control group no,inflammation and in the Apexit group and Grossman's group moderate inflammation and in the Apadana's sealer group moderate to severe inflammation were seen which were statistically significant.
In the 60th day, there was no inflammation in the control group. In Grossman's sealer group mild inflammation and in Apexit's sealer group mile to moderate inflammation were observed. In statistical manner, there is no significant difference between the Apadana's Roth sealer and Apexit's sealer but between these two sealers and Grossman's and the control group, there are significant statistical difference with P=0.008.