Introduction: Helicobacter pylori is a gram negative bacterium which has adapted to the ecological conditions of gastric mucosa. Due to its specific characteristics, H.pylori can cause the onset and duration of a chronic damage of gastric mucosa. Method & Material: To assess the value of touch (imprint) cytology in the diagnosis of helicobacter pylori infection of gastric mucosa, four touch smears were prepared from two antral biopsy specimens, obtained from a total of 100 patients with various GT complaints who underwent upper GI endoscopy in Shahid Mohammadi hospital of Bandar Abbas. The smears were stained using wright, giemsa, papanicolaou and gram methods. The results, expressed as the presence or absence of H.P micro organisms, were compared with histopathologic examination and rapid urease test as two gold standard methods separately. Results: In comparison with histopathology, wright method had the most sensitivity, specificity, positive and negative predictive values and efficiency. Efficiency range of these four methods was between 72% and 80%. In comparison with golden standard (urease test), wright method had the most sensitivity, negative predictive value and efficiency, whereas, gram method had the most specificity and positive predictive value. Wright, gram and gimsa methods in comparison with gold standard, had efficiency of 83% and papanicolaou had efficiency of 79%. Conclusion: According to above results, imprint smears, stained using each of the four above mentioned staining methods is recommended as a quick, simple and cost-effective test.